小麦田大穗看麦娘对精噁唑禾草灵的抗性  

Resistance of Alopecurus myosuroides in wheat against fenoxaprop-P-ethyl

作　　者：葛鲁安 朱宝林 赵宁[1] 赵孔平 王恒智[1] 刘伟堂[1] 王金信[1] GE Lu’an;ZHU Baolin;ZHAO Ning;ZHAO Kongping;WANG Hengzhi;LIU Weitang;WANG Jinxin(College of Plant Protection,Shandong Agricultural University,Tai'an 271018,Shandong Province,China)

机构地区：[1]山东农业大学植物保护学院,山东泰安271018

出　　处：《农药学学报》2020年第1期82-87,共6页Chinese Journal of Pesticide Science

基　　金：国家自然科学基金面上项目(31772181);山东省重大科技创新工程项目(2018CXGC0213);山东省“双一流”建设专项(SYL2017XTTD11).

摘　　要：为明确小麦田大穗看麦娘对精噁唑禾草灵的抗性水平及产生抗性的机理,采用整株法测定了河南省小麦田大穗看麦娘种群对精噁唑禾草灵的抗性水平,以及细胞色素P450s抑制剂胡椒基丁醚(PBO)对精噁唑禾草灵的增效作用,并通过基因测序技术研究了其靶标ACCase基因的突变位点。结果显示:与敏感种群HN-06相比,抗性种群HN-05对精噁唑禾草灵的抗性倍数为52.2,其ACCase基因存在Ile-2041-Asn和Gly-2096-Ala位点突变;喷施PBO后,精噁唑禾草灵对大穗看麦娘的GR50值(有效成分)为5.4 g/hm^2,表现出明显的增效作用,与未喷施PBO处理的差异倍数为161.3。研究表明,抗性种群HN-05对精噁唑禾草灵已产生高水平抗性,该抗性的产生可能是由于其靶标基因突变和P450s介导的代谢增强同时导致的,即表现出了靶标抗性和非靶标抗性共存的现象。The resistant level, mechainism of resistance of Alopecurus myosuroides against fenoxapropP-ethyl were determined. Results of the whole-plant dose-response test showed that population HN-05 from Henan Province had evolved high-level resistance against fenoxaprop-P-ethyl(52.2-fold). Using the gene sequencing method, two amino acid mutations at codon 2041(Ile-Asn) and 2096(Gly-Ala)were detected in the ACCase gene of the resistant population HN-05. Pre-treatment with the known metabolic inhibitor, piperonyl butoxide(PBO), enhanced the activity of fenoxaprop-P-ethyl(161.3-fold). So that the GR50 value(active ingredient) of fenoxaprop-P-ethyl to HN-05 is 5.4 g/hm^2. This study confirmed the resistance of A. myosuroides against the ACCase inhibitor, fenoxaprop-P-ethyl.The resistance mechanism were conferred by specific ACCase point mutations at amino acid position Ile-2041-Asn and Gly-2096-Ala and P450 s-based metabolism.

关 键 词：大穗看麦娘 精噁唑禾草灵 靶标抗性 非靶标抗性 乙酰辅酶A羧化酶 细胞色素P450酶系 

分 类 号：S482.4[农业科学—农药学] S481.4[农业科学—植物保护]