UPLC Q-Exactive Orbitrap MS分析栀子水提液中的化学成分  

Identification of Chemical Components of Gardenia Water Extract by UPLC Q-Exactive Orbitrap MS

作　　者：何理想 王允吉 张倩倩 冯芳[1,2] HE Li-xiang;WANG Yun-ji;ZHANG Qian-qian;FENG Fang(Department of Pharmaceutical Analysis,China Pharmaceutical University,Jiangsu Nanjing 210009;Key Laboratory of Drug Quality Control and Pharmacovigilance(Ministry of Education),Jiangsu Nanjing 210009,China)

机构地区：[1]中国药科大学药物分析教研室,江苏南京210009 [2]药物质量与安全预警教育部重点实验室,江苏南京210009

出　　处：《广州化工》2020年第5期94-97,共4页GuangZhou Chemical Industry

基　　金：国家自然科学基金资助项目(No:81872993)。

摘　　要：利用超高效液相色谱-四级杆-静电场轨道阱联用技术(UPLC-Q-Exactive Orbitrap-MS)得到各成分精确的分子量和二级质谱碎片信息,对栀子水提液中的成分进行结构推测和鉴定。采用ACQUITY UPLC BEH C18色谱柱(150 mm×2.1 mm,1.7μm),以乙腈-0.1%甲酸水溶液进行梯度洗脱,流速:0.3 mL/min,柱温:40℃。质谱采用电喷雾离子源(ESI),正负离子监测模式。结果从栀子水提液中检测出了54种化学成分,鉴定了44种化学成分的结构,为进一步研究栀子的药理活性和临床用药奠定物质基础。The accurate molecular weight and secondary mass spectrum fragment information of each component were obtained by UPLC-Q-Exactive Orbitrap-MS,and the components in gardenia water extract were predicted and identified.ACQUITY UPLC BEH C18 column(150 mm×2.1 mm,1.7μm)was used,mobile phase was gradient elution with 0.1%formic-acetonitrile,flow rate was 0.3 mL/min,and column temperature was 40℃.Mass spectrometry was performed by using electrospray ion source(ESI)under the positive and negative ion modes.54 chemical components were detected from the water extract of gardenia,and 44 chemical components were identified,which could lay a material foundation for the further study of the pharmacological activity and clinical drug use of gardenia.

关 键 词：栀子提取液 UPLC-Q-Exactive Orbitrap-MS 化学成分 

分 类 号：R917[医药卫生—药物分析学]