复合固定化脂肪酶催化餐厨废弃油脂合成生物柴油  

Synthesis of biodiesel from waste cooking oil using a compound of immobilized lipases

作　　者：杨建斌 YANG Jian-bin(Shanghai Zhongqi Environment Technology Co.,Ltd.,Shanghai 201419,China)

机构地区：[1]上海中器环保科技有限公司,上海201419

出　　处：《精细与专用化学品》2020年第2期5-9,共5页Fine and Specialty Chemicals

摘　　要：探讨了复合固定化脂肪酶(Rhizopus oryzae lipase和Candida rugosa lipase)催化餐厨废弃油脂合成生物柴油的工艺条件。实验结果表明,单独使用1,3位专一性脂肪酶R.oryzae lipase催化餐厨废弃油脂,反应18h,生物柴油转化率达到70%;单独使用非专一性脂肪酶C.rugosa lipase,反应30h,生物柴油转化率可达20%。为了更有效提高生物柴油转化率,采用1,3位专一性脂肪酶R.oryzae lipase和非专一性脂肪酶C.rugosa lipase复合固定化脂肪酶催化合成生物柴油,反应21h,生物柴油转化率可达到96.5%。同时对该复合酶的稳定性进行了实验,在连续催化反应10个批次(300h)后,生物柴油转化率仍保持在80%以上。Biodiesel was synthesized with waste cooking oil as raw materials and using a compound of immobilized R.oryzae and C.rugosa lipases as catalyst.Technology conditions were investigated.Biodiesel conversion from waste cooking oil reached a maximum of 70% at 18 h using immobilized 1,3-specific R.oryzae lipase alone.Biodiesel conversion failed to reach 20% after 30 h when immobilized nonspecific C,Rugosa lipase alone was used.To increase the biodiesel production yield,a mixture of immobilized 1,3-specific R.oryzae lipase and nonspecific C.rugosa lipase was used.The biodiesel conversion of 96.5% was reached using the mixture at 21 h.When the stability of the immobilized lipases mixture was tested,biodiesel conversion was maintained at over 80% of its original conversion after 10 cycles.

关 键 词：生物柴油 餐厨废弃油脂 复合脂肪酶 固定化 稳定性 

分 类 号：TQ225[化学工程—有机化工]