不同唐古特大黄提取物对高脂模型大鼠血脂及动脉粥样硬化的影响  

Effect of Different Tanggute Dahuang (Rheum Tanguticum) Extracts on Serum Lipid and Atherosclerosis in Hyperlipemia Rats

作　　者：杨昊虹 罗巧玉[1] 祁得胜 马银花 张勐 王琳璇 谢惠春[1] YANG Haohong;LUO Qiaoyu;QI Desheng;MA Yinhua;ZHANG Meng;WANG Linxuan;XIE Huichun(Qinghai Normal University/Qinghai Provincial Key Laboratory of Medicinal Animal and Plant Resources,Xining Qinghai China 810008)

机构地区：[1]青海师范大学/青藏高原药用动植物资源青海省重点实验室,青海西宁810008

出　　处：《中医学报》2020年第2期338-343,共6页Acta Chinese Medicine

基　　金：青海省自然科学基金项目(2017-ZJ-774);中国科学院2018年度“西部之光”人才培养引进计划项目(人字[2018]99号);青海省青藏高原药用动植物资源重点实验室项目(2017-ZJ-Y13)。

摘　　要：目的:观察唐古特大黄醇提物和水提物对高脂模型大鼠血脂、肝脏及动脉粥样硬化的影响。方法:54只大鼠随机分为正常对照组(6只)和造模组(48只)。正常对照组饲喂基础饲料,造模组大鼠一次性腹腔注射维生素D3(300 000 IU·kg^-1)后,每日饲以高脂饲料,并于每周灌胃维生素D3粉剂(饲料级)75 000 IU·kg^-1。造模成功后,将模型大鼠分为高脂模型对照组,辛伐他汀组,水提物高剂量组、中剂量组、低剂量组,醇提物高剂量组、中剂量组、低剂量组,每组6只。各组给予相应的试药,7周后比较各组大鼠血脂指标,观察大鼠主动脉及肝脏形态学变化。结果:与高脂模型组比较,给药各组大鼠总胆固醇、三酰甘油、低密度脂蛋白胆固醇均不同程度降低,尤以水提高剂量组变化显著(P<0.05)。给药各组高密度脂蛋白胆固醇水平高于模型组,以醇提高剂量组差异最为明显(P<0.05)。给药组肝脏、主动脉病变程度较模型组明显减轻,其中以醇提高剂量组效果最佳。结论:唐古特大黄能够有效降低大鼠血脂并干预动脉粥样硬化的形成,大黄水提物在降血脂方面效果更佳,大黄醇提物则在改善肝脏、主动脉病变方面呈现出优越性。Objective:To observe the effects of rheum tanguticum extract and water extract on blood lipid,liver and atherosclerosis in high-fat model rats.Methods:54 rats were randomly divided into normal control group(6 rats)and model group(48 rats).The normal control group was fed with basic feed,and the rats in the model group were injected intra-peritoneally with vitamin D3(300 000 IU·kg^-1),then fed with high-fat diet daily,and administrated vitamin D3 powder(feed grade)weekly.75 000 IU·kg^-1.After successful modeling,the model rats were divided into a high-fat model control group,a simvastatin group,a water extract high-dose group,a medium-dose group,a low-dose group,an alcohol-extract high-dose group,a medium-dose group,and a low-dose group,with 6 rats in each group.Corresponding test drugs were given to each group.After 7 weeks,blood lipid indexes of rats in each group were compared,and morphological changes of aorta and liver in rats were observed.Results:Compared with the high-fat model group,the total cholesterol,triacylglycerol,and low-density lipoprotein cholesterol of the rats in each group were reduced to varying degrees,especially in the water-increased dose group(P<0.05).High-density lipoprotein cholesterol levels in each group were higher than those in the model group,and the difference was most obvious in the alcohol-raised dose group(P<0.05).The degree of liver and aortic lesions in the administration group was significantly reduced compared with the model group,and the effect of increasing the dose with alcohol was the best.Conclusion:rheum tanguticum extract can effectively reduce blood lipids and interfere with the formation of atherosclerosis in rats.Rhubarb water extracts are more effective in lowering blood lipids,and rhubarb alcohol extracts are superior in improving liver and aortic lesions.

关 键 词：唐古特大黄水提取物 唐古特大黄醇提取物 血脂 动脉粥样硬化 大鼠 

分 类 号：R285.5[医药卫生—中药学]