茵陈五苓散对非酒精性脂肪性肝病模型大鼠RBP4的影响  

Effect of Yinchen Wuling Powder on RBP4 in Non-alcoholic Fatty Liver Disease Model Rats

作　　者：谭婷 何栋[1] 朱红梅 田秋慧 徐晴 欧阳心怡 戴一天 艾碧琛[1] TAN Ting;HE Dong;ZHU Hongmei;TIAN Qiuhui;XU Qing;OUYANG Xinyi;DAI Yitian;AI Bichen(Hunan University of Chinese Medicine,Changsha Hunan China 410000)

机构地区：[1]湖南中医药大学,湖南长沙410000

出　　处：《中医学报》2020年第2期344-347,共4页Acta Chinese Medicine

基　　金：湖南省教育厅重点项目(12A101);湖南中医药大学大学生创新项目(1022/0001019010)。

摘　　要：目的:观察RBP4在非酒精性脂肪性肝病模型大鼠中的表达,并探讨茵陈五苓散对非酒精性脂肪肝病的治疗效果及作用机制。方法:60只SD大鼠随机分为空白对照组(15只)与实验组(45只)。空白对照组给予普通饲料,实验组大鼠饲喂改良以后的高脂饲料,制作非酒精性脂肪肝病模型,饲喂24周。造模成功后,将实验组的大鼠随机分为4组,非酒精性脂肪肝组、茵陈五苓散1.28 g·mL^-1组、茵陈五苓散0.64 g·mL^-1组和辛伐他汀对照组。每组大鼠进行灌胃,给予对应的药物治疗,6周后比较各组大鼠肝质量、体质量、肝指数、低密度脂蛋白胆固醇(low density lipoprotein cholesterin,LDL-C)、三酰甘油(triacylglycerol,TG)、总胆固醇(total cholesterol,TC)水平,采用HE染色,观察肝脏病理形态学的改变;ELISA法检测肝组织中RBP4的表达。结果:与空白对照组比较,非酒精性脂肪肝组大鼠的TG、LDL-C、体质量、肝指数、肝质量、TC、RBP4的表达均升高(P<0.01)。与非酒精性脂肪肝组比较,辛伐他汀对照组、茵陈五苓散1.28 g·mL^-1组和茵陈五苓散0.64 g·mL^-1组大鼠各指标的表达均降低(P<0.01)。与辛伐他汀对照组比较,茵陈五苓散1.28 g·m L^-1组各指标均降低(P<0.01),茵陈五苓散0.64 g·mL^-1组除体质量外,其余各指标均降低(P<0.01)。与模型组比较,各治疗组肝组织病理变化改善,且茵陈五苓散1.28 g·mL^-1组的改善结果优于茵陈五苓散0.64 g·mL^-1组和辛伐他汀组。结论:茵陈五苓散可通过调节大鼠RBP4的表达水平,改善脂代谢,减缓脂肪变性程度,从而抑制脂质过氧化反应和减少细胞变性,进而保护肝组织,治疗非酒精性脂肪性肝病。Objective:To observe the expression of RBP4 in non-alcoholic fatty liver disease model rats,and to explore the therapeutic effect and mechanism of Yinchen Wuling Powder on non-alcoholic fatty liver disease.Methods:Sixty SD rats were randomly divided into blank control group(15)and experimental group(45).The blank control group was given ordinary feed,and the experimental group rats were fed with modified high-fat feed to make a model of non-alcoholic fatty liver disease and fed for 24 weeks.After successful modeling,the rats in the experimental group were randomly divided into 4 groups:non-alcoholic fatty liver group,Yinchen Wuling Powder 1.28 g·mL^-1group,Yinchen Wuling Powder 0.64 g·m L^-1group,and simvastatin control group.Rats in each group were administrated with gastrointestinal administration,and the corresponding drugs were given.After 6 weeks,the liver quality,body weight,liver index,low density lipoprotein cholesterin(LDL-C),and triacylglycerol were compared in each group(triacylglycerol,TG),total cholesterol(TC)levels,HE staining was used to observe the pathological and morphological changes of the liver;ELISA was used to detect the expression of RBP4 in liver tissue.Results:Compared with the blank control group,the expressions of TG,LDL-C,body weight,liver index,liver quality,TC,and RBP4 in non-alcoholic fatty liver group rats were increased(P<0.01).Compared with the non-alcoholic fatty liver group,the expressions of all indicators in the simvastatin control group,Yinchen Wuling Powder 1.28 g·mL^-1group and Yinchen Wuling Powder 0.64 g·mL^-1group were all reduced(P<0.01).Compared with the simvastatin control group,the results of Yinchen Wuling Powder 1.28 g·m L^-1group and Yinchen Wuling Powder 0.64 g·mL^-1group were all reduced(P<0.01).Compared with the model group,the pathological changes of liver tissue in each treatment group were improved,and the improvement of Yinchen Wuling Powder 1.28 g·mL^-1group was better than that of Yinchen Wuling Powder 0.64 g·mL^-1group and simvastatin group.Con

关 键 词：茵陈五苓散 非酒精性脂肪性肝病 RBP4 低密度脂蛋白胆固醇 三酰甘油 总胆固醇 大鼠 

分 类 号：R285.5[医药卫生—中药学]