黄连半夏配伍对高脂血症模型大鼠血脂的影响  

Effect of Huanglian(Rhizoma Coptidis)Combined with Banxia(Rhizoma Pinelliae)on Blood Lipid in Hyperlipidemia Model Rats

作　　者：丁劭华 张卫华[1] 睢瑞卿 冯鸣 罗斌 DING Shaohua;ZHANG Weihua;SUI Ruiqing;FENG Ming;LUO Bin(Nanjing University of Chinese Medicine,Nanjing Jiangsu China 210023)

机构地区：[1]南京中医药大学,江苏南京210023

出　　处：《中医学报》2020年第3期608-612,共5页Acta Chinese Medicine

基　　金：江苏省高校优势学科建设工程资助项目(PAPD);江苏高校境外研修计划资助项目{苏教师[2015]35号}。

摘　　要：目的:观察黄连半夏配伍对高脂血症模型大鼠血脂的调节作用及可能机制。方法:健康SD雄性大鼠60只随机分为空白对照组、模型组、阳性对照组(辛伐他汀组)、黄连半夏配伍高剂量组、黄连半夏配伍中剂量组、黄连半夏配伍低剂量组,每组10只。空白对照组给予普通饲料喂养,其他各组给予高脂饲料,自由饮水,连续3周。造模成功后,辛伐他汀组给予辛伐他汀0.01 g·kg^-1,黄连半夏配伍低剂量组、中剂量组、高剂量组分别给予剂量为3、6、12 g·kg^-1(按生药计),空白对照组给予等体积的生理盐水,每日1次,连续8周。给药期间,模型组、辛伐他汀组、中药各治疗组继续给予高脂饲料喂养。末次给药后腹主动脉采血,检测相应指标。结果:与空白组比较,模型组肝质量及肝质量/体质量、脂肪系数、总胆固醇(total cholesterol,TC)、三酰甘油(triglyceride,TG)、低密度脂蛋白胆固醇(low density lipoprotein cholesterol,LDL-C)、白细胞介素-1β(interleukin-1β,IL-1β)、IL-6、肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、单核细胞趋化因子-1(monocyte chemoattractant protein-1,MCP-1)、核转录因子-κB(nuclear transcription factor-κB,NF-κB)、载脂蛋白B100(apolipoproteinB100,ApoB100)显著升高(P<0.01),高密度脂蛋白胆固醇(high density lipoprotein cholesterol,HDL-C)、载脂蛋白AI(apolipoprotein AI,ApoAI)、低密度脂蛋白受体(low density lipoprotein receptor,LDLR)、固醇调节元件结合蛋白-2(sterol regulatory element binding protein-2,SREBP-2)显著降低(P<0.01);与模型组比较,辛伐他汀组、中药配伍各组能够不同程度地降低肝质量及肝质量/体质量、脂肪系数、TC、TG、LDL-C、IL-1β、IL-6、TNF-α、MCP-1、NF-κB、ApoB100(P<0.01或P<0.05),升高ApoAI、LDLR、SREBP-2(P<0.01或P<0.05)。其中,黄连半夏配伍高剂量组作用更为显著。结论:黄连半夏配伍对高脂血症大鼠有明显的降血脂作用,其机制可能与Objective:To observe the regulatory effect of Huanglian(Rhizoma Coptidis)combined with Banxia(Rhizoma Pinelliae)on blood lipid in hyperlipidemia model rats and its possible mechanism.Methods:60 healthy SD male rats were randomly divided into blank control group,model group,positive control group(simvastatin group),high dose Huanglian(Rhizoma Coptidis)combined with Banxia(Rhizoma Pinelliae)group,middle dose Huanglian(Rhizoma Coptidis)combined with Banxia(Rhizoma Pinelliae)group and low dose Huanglian(Rhizoma Coptidis)combined with Banxia(Rhizoma Pinelliae)group,10 rats in each group.The blank control group was fed with common diet,the other groups were fed with high-fat diet and free drinking water for 3 weeks.After the model was established successfully,simvastatin group was given 0.01 g·kg^-1 simvastatin,Huanglian(Rhizoma Coptidis)combined with Banxia(Rhizoma Pinelliae)low dose group,middle dose group and high dose group were given 3,6 and 12 g·kg^-1 respectively(according to the amount of raw herbs),and blank control group was given equal volume of normal saline once a day for 8 weeks.During the period of administration,the model group,simvastatin group and each treatment group of Chinese medicine continued to be fed with high-fat diet.Blood was collected from the abdominal aorta after the last administration and the corresponding indexes were detected.Results:Compared with the blank group,the model group had liver mass,liver mass/body mass,fat coefficient,total cholesterol(TC),triglyceride(TG),low density lipoprotein cholesterol(LDL-C),interleukin-1β(IL-1β),IL-6,tumor necrosis factor-α(TNF-α),monocyte chemoattractant protein-1(MCP-1),nuclear transcription factor-κB(NF-κB),apolipoproteinB100(ApoB100)significantly increased(P<0.01),high density lipoprotein cholesterol(HDL-C),apolipoprotein AI(ApoAI),low density lipoprotein receptor(LDLR),sterol regulatory element binding protein-2(SREBP-2)decreased significantly(P<0.01);compared with the model group,simvastatin group and Chinese medicine compatibility gr

关 键 词：黄连 半夏 配伍 高脂血症 脂代谢 血脂 炎症因子 核转录因子-κB 载脂蛋白AI 固醇调节元件结合蛋白-2 大鼠 

分 类 号：R285.5[医药卫生—中药学]