地黄提取物对人PC-3细胞凋亡机制的影响  

Efficacy of the the Dihuang extract on mechanism of apoptosis in human PC-3 cells

作　　者：孙继建 崔洪泉[1] 潘世杰[1] 陈瑞廷 王国桥 Sun Jijian

机构地区：[1]河南中医药大学第二附属医院,河南郑州450002

出　　处：《中医临床研究》2020年第2期1-3,共3页Clinical Journal Of Chinese Medicine

基　　金：河南省中医药专项课题研究项目,项目编号:2017ZY3006;河南省中医药科学研究专项课题:项目编号:2019ZY2043。

摘　　要：目的:探讨熟地黄乙醇提取物对人前列腺癌PC-3细胞凋亡,初步探讨其作用的分子机制。方法:不同浓度(2.0、2.5、3.0、3.5 mg/mL)的熟地黄乙醇提取物作用于人前列腺癌PC-3细胞24 h、48 h后,采用噻唑蓝(Methyl Thiazolyl Tetrazolium,MTT)法检测熟地黄乙醇提取物对前列腺癌PC-3细胞分裂增殖的影响;Western-bloting法检测Livin、Survivin、Caspase3凋亡相关蛋白的表达。结果:抑制率具有浓度时间依赖性;熟地黄乙醇提取物能够影响PC-3细胞凋亡相关蛋白Livin、Survivin、Caspase3的表达。结论:熟地黄乙醇提取物抑制前列腺癌细胞增殖的机制可能与诱导前列腺癌细胞凋亡以及影响其凋亡相关蛋白的表达有关。Objective:To explore efficacy of ethanol extract of rehmannia glutinosa on the apoptosis of human prostate cancer PC-3 cells and the molecular mechanism.Methods:The ethanol extracts of rehmannia glutinosa at different concentrations(2.0,2.5,3.0,3.5 mg/mL)were applied in human prostate cancer PC-3 cells for 24 and 48 hours.Efficacy of ethanol extracts on the proliferation and proliferation of prostate cancer PC-3 cells was detected by MTT.Western-bloting method was applied to detect the expression of Livin,Survivin,and Caspase3 apoptosis-related proteins.Results:The inhibition rate showed concentration-and time-dependent.The ethanol extract of rehmannia glutinosa could affect the expression of apoptosis-related proteins Livin,Survivin,and Caspase3 in PC-3 cells.Conclusion:The mechanism of ethanol extract from rehmannia glutinosa on inhibiting the proliferation of prostate cancer cells may be related to the induction of apoptosis of prostate cancer cells and the expression of apoptosis-related proteins.

关 键 词：熟地黄 前列腺癌 PC-3细胞 凋亡机制 

分 类 号：R329.28[医药卫生—人体解剖和组织胚胎学]