银纳米基比色传感法测定水样中汞离子的方法  

Determination of Mercury Ions in Water Samples by Silver Nanoparticle Colorimetric Sensor

作　　者：魏佳 徐海星 顾翔源 杨泽怡 陆姗姗 李欣怡[1] 蒋依轩 孔雨嘉 朱颖越[1] WEI Jia;XU Haixing;GU Xiangyuan;YANG Zeyi;LU Shanshan;LI Xinyi;JIANG Yixuan;KONG Yujia;ZHU Yingyue(School of Biology and Food Engineering,Changshu Institute of Technology,Changshu 215500,China)

机构地区：[1]常熟理工学院生物与食品工程学院,江苏常熟215500

出　　处：《常熟理工学院学报》2020年第2期103-107,共5页Journal of Changshu Institute of Technology

基　　金：国家自然科学基金项目“石墨稀/贵金属有序自组装复合纳米材料及其光学特性”(51602029);苏州市科技计划项目“农产品中重金属污染直接非竞争法快速检测及其增敏新方法研究”(SNG201617);江苏省“六大人才高峰”第十二批高层次人才选拔培养项目“食品中重金属污染快速超灵敏检测技术的研究”(2015-NY-021);江苏省“青蓝工程”优秀青年骨干教师培养对象项目(2018)。

摘　　要：基于银纳米粒子在盐诱导下聚集使体系颜色发生改变这一原理,建立一种生物传感方法检测水样中的汞离子.采用柠檬酸还原法合成银纳米粒子并修饰以核酸适配体,汞离子与核酸适配体结合发生变构效应导致银纳米粒子暴露在盐环境中发生聚集,使体系光谱特征峰和颜色发生明显变化.研究体系特定波长吸光度比值与汞离子浓度的变化关系.在最佳优化条件下,该比色法在质量浓度区间为0.2~50 ng/mL呈现良好线性关系,检出限为0.11 ng/mL.将该检测体系应用于汞离子类似物的检测,特异性较好.该方法对水样中汞离子的检测有一定的应用前景.A method based on the aggregation of silver nanoparticles under salt induction to change the color of the system was studied to establish a biosensor to detect mercury ions in water samples.Silver nanoparticles were synthesized by citric acid reduction method and modified with nucleic acid aptamers.The allosteric effect of mercury ions combined with nucleic acid aptamers resulted in the aggregation of silver nanoparticles in the salt environment,which made the spectral peaks and colors of the system change obviously.This paper also made a study of the relationship between the specific wavelength absorbance ratio and the mercury ion concentration.Under the optimal optimization conditions,the colorimetric method showed a good linear relationship in the mass concentration range of 0.2-50 ng/mL,and the detection limit was 0.11 ng/mL.Therefore,the detection system can be applied to the detection of mercury ion analogs with good specificity.This method has certain application prospects for the detection of mercury ions in water samples.

关 键 词：汞离子 银纳米粒子 核酸适配体 吸光度 

分 类 号：S835.2[农业科学—畜牧学]