高效液相色谱法测定化妆品中香豆素类化合物  

Determination of coumarins in cosmetics by high performance liquid chromatography

作　　者：刘国清 李静[2] 许建帼 吕延文 潘向军 董云渊 LIU Guo-qing;LI Jing;XU Jian-guo;LV Yan-wen;PAN Xiang-jun;DONG Yun-yuan(College of Chemical and Material Engineering,Quzhou University,Quzhou,Zhejiang 324000,China;Department of Chemical&Biological Engineering,Zhejiang University,Hangzhou,Zhejiang 310027,China)

机构地区：[1]衢州学院化学与材料工程学院,浙江衢州324000 [2]浙江大学化学工程与生物工程学院,浙江杭州310027

出　　处：《日用化学工业》2020年第3期204-207,212,共5页China Surfactant Detergent & Cosmetics

基　　金：“十三五”国家重点研发计划专项资助(2017YFB0308200)。

摘　　要：建立了化妆品中8-羟基补骨脂素、补骨脂素、异补骨脂素、8-甲氧基补骨脂素、5-甲氧基补骨脂素、三甲沙林、欧前胡素、异欧前胡素8种香豆素类化合物含量鉴定的高效液相色谱分析方法。样品经超临界CO2萃取法提取,其中以甲醇作为载体助溶剂。采用Agilent ZORBAX Eclipse Plus C18色谱柱(250 mm×4.6 mm×5μm)作为分析柱,乙腈和水作为二元流动相,柱温25℃,流速1.0 mL/min,检测波长250 nm,外标法定量。结果表明,8种化合物的线性相关系数均大于0.999,低、中、高3种加标水平下的平均加标回收率为85.1%~110.0%,相对标准偏差RSD<6.42%(n=6)。该方法简单、高效、准确,为化妆品中香豆素类化合物定量提供了一种有效的分析技术。A high performance liquid chromatographic(HPLC)method was developed for the simultaneous determination of 8 coumarin compounds in cosmetics,including 8-hydroxypsoralen,psoralen,isopsoralen,8-methoxypsoralen,5-methoxypsoralen,trioxsalen,imperatorin and isoimperatorin.The samples were extracted by supercritical CO2 extraction with methanol as carrier cosolvent.Chromatographic separation was achieved on an Agilent ZORBAX Eclipse Plus C18 chromatographic column(250 mm×4.6 mm×5μm,column temperature 25℃)through gradient elution with acetonitrile-water as binary mobile phase at a flow rate of 1.0 mL/min,and the detection wavelength was 250 nm.The external standard method was used for quantification.The results showed that the linear correlation coefficients of the 8 compounds were all greater than 0.999;the average recoveries at three spiked levels were in the range of 85.1%-110.0%with RSDs(n=6)lower than 6.42%.This method was proved to be simple,efficient and accurate,which provided an effective detection technique for coumarin compounds in cosmetics.

关 键 词：化妆品 香豆素类化合物 高效液相色谱 超临界CO2萃取 

分 类 号：TQ658[化学工程—精细化工]