替米沙坦和氯沙坦对胰岛素抵抗大鼠冠状动脉脂联素受体和胰岛素抵抗的影响  

The Influence of Telmisartan and Losartan on Adiponectin Receptors in Rats Coronary Arteries with Insulin Resistance

作　　者：沈絮华[1] 邱惠[1] 梁思文[1] SHEN Xuhua;QIU Hui;LIANG Siwen(Beijing Friendship Hospital,Capital Medical University,Beijing 100050,China)

机构地区：[1]首都医科大学附属北京友谊医院,北京100050

出　　处：《中西医结合心脑血管病杂志》2020年第4期572-575,共4页Chinese Journal of Integrative Medicine on Cardio-Cerebrovascular Disease

摘　　要：目的探讨替米沙坦和氯沙坦对胰岛素抵抗(IR)大鼠冠状动脉脂联素受体和IR的影响。方法将40只雄性SD大鼠随机分为对照组(Con组)、胰岛素抵抗组(IR组)、替米沙坦干预组[TEL组,5 mg/(kg·d)]和氯沙坦干预组[LOS组,10 mg/(kg·d)]。测定并比较各组大鼠空腹血糖、总胆固醇(TC)、三酰甘油(TG)和空腹胰岛素、血清脂联素(APN),并计算胰岛素敏感指数(ISI)。检测冠状动脉APN、脂联素受体1(AdipoR1)和脂联素受体2(AdipoR2)mRNA和蛋白的表达。结果与IR组比较,TEL组和LOS组血清APN水平均增加(P<0.05),且TEL组TG水平、空腹血糖和ISI低于IR组(P<0.05)。IR组冠状动脉APN mRNA、AdipoR1 mRNA、AdipoR2 mRNA表达低于Con组(P<0.05),TEL组和LOS组冠状动脉APN mRNA、AdipoR1 mRNA、AdipoR2 mRNA表达较IR组增高(P<0.05)。与IR组比较,TEL组和LOS组冠状动脉APN、AdipoR1和AdipoR2表达增加(P<0.05)。结论替米沙坦和氯沙坦均能增加IR大鼠血清APN水平,抑制冠状动脉APN、AdipoR1和AdipoR2表达的下降。Objective To explore the influence of telmisartan and losartan on adiponectin(APN)receptors(AdipoR)in rats with insulin resistance(IR).Methods Forty male sprague-dawley(SD)rats were randomly divided into control group(Con group),insulin resistance group(IR group),telmisartan intervention group[5 mg/(kg·d),TEL group]and losartan intervention group[10 mg/(kg·d),LOS group].Blood pressure(BP),APN,fasting blood glucose(FBG),fasting serum insulin(FSI),triglyceride(TG),total cholesterol(TC),and ISI were observed.The mRNA and protein expressions of AdipoR1,AdipoR2,and APN in rats coronary arteries(RCAs)were detected by reverse transcription-polymerase chain reaction(RT-PCR)and Western blot.Results Compared with Con group,the mRNA and protein expressions of AdipoR1,AdipoR2,and APN in IR group were significantly decreased(P<0.05).Compared with IR group,APN in TEL group and LOS group were significantly increased(P<0.05),the levels of TG and FBG in TEL group were decreased(P<0.05),ISI was improved(P<0.05).The expression of APN,AdipoR1,and AdipoR2 in TEL group and LOS group were increased(P<0.05).Conclusion Telmisartan and losartan could increase the levels of APN and inhibit the expressions of APN,AdipoR1,and AdipoR2 in the coronary artery of IR rats.

关 键 词：胰岛素抵抗 替米沙坦 氯沙坦 脂联素 冠状动脉 大鼠 

分 类 号：R589[医药卫生—内分泌] R259[医药卫生—内科学]