甘西鼠尾草对大鼠高原肺动脉高压的干预作用及机制  

Effects of Salvia przewalskii Maxim.on high-altitude pulmonary hypertension in rats and its mechanism

作　　者：王亚峰[1] 王爱霞 王生彪 朵德龙[1] 李茜 严英俊 WANG Ya-feng;WANG Ai-xia;WANG Sheng-biao;DUO De-long;LI Qian;YAN Ying-jun(PIVAS of QingHai Provincial People’s Hospital,Xining 810007,China)

机构地区：[1]青海省人民医院静配中心,西宁810007

出　　处：《中国应用生理学杂志》2019年第6期533-536,共4页Chinese Journal of Applied Physiology

基　　金：青海省科技厅基金资助项目(2018—ZJ—786)。

摘　　要：目的:探讨甘西鼠尾草(SPM)对大鼠高原肺动脉高压(HAPH)的干预作用及可能的机制。方法:将雄性SD大鼠随机分成对照组、缺氧组、SPM(0.5 g/kg、1 g/kg、2 g/kg)剂量组,每组14只,对照组饲养于西宁(海拔约2260 m),其余组均饲养于玛多县人民医院(海拔约4260 m)。SPM剂量组灌胃不同浓度的SPM(1 ml/100 g),浓度分别为0.5 g/kg、1 g/kg、2 g/kg,对照组和缺氧组灌胃等体积蒸馏水,每日一次,连续4周后,测定大鼠平均肺动脉压(mPAP)并取相同部位肺组织置液氮保存备用。采用RT-PCR法测定每组大鼠肺组织中的细胞增殖核抗原(PCNA)、细胞周期素依赖激酶(CDK4)、细胞周期蛋白D(CyclinD1)、RhoA(Ras同源基因家族成员A)、ROCK1、ROCK2的mRNA表达水平。结果:与对照组比较,缺氧组大鼠mPAP、肺组织中PCNA、CDK4、CyclinD1、RhoA、ROCK1、ROCK2的mRNA表达水平均明显升高(P<0.01)。与缺氧组比较,SPM剂量组大鼠的mPAP、肺组织中PCNA、CDK4、CyclinD1、RhoA、ROCK1、ROCK2的mRNA表达水平均明显降低(P<0.05或P<0.01)。结论:SPM对大鼠HAPH具有一定的预防作用,其机制可能与抑制肺动脉平滑肌细胞过度增殖和RhoA/Rho激酶(ROCK)信号通路过度激活有关。Objective:To investigate the interventive effects of Salvia przewalskii Maxim.(SPM)on high-altitude pulmonary hypertension(HAPH)in rats and possible mechanism.Methods:The male SD rats were randomly divided into the control group,the hypoxia group and SPM(0.5 g/kg,1 g/kg and 2 g/kg)group.There were 14 rats in each group.The rats in control group were feed in Xining(with an altitude about 2260 m),and the other group rats were all feed in Maduo county people’s hospital(with an altitude about 4260 m).The rats in SPM groups were treated with SPM at the doses of 0.5 g/kg,1 g/kg and 2 g/kg by gavage respectively(100 g/ml).The rats in control and the hypoxia groups were received equal volume of distilled water,once a day.After 4 weeks,the mean pulmonary artery pressure(mPAP)of rats was measured and the same part of lung tissue of each rat was collected and stored in liquid nitrogen.Then the relative mRNA expression levels of the proliferation cell nuclear antigen(PCNA),the cell cycle dependent kinase 4(CDK4),CyclinD1,RhoA,ROCK1,ROCK2 in lung tissues of each group rats were all tested by RT-PCR.Results:Compared with the control group,the mPAP and the relative mRNA expression levels of PCNA,CDK4,CyclinD1,RhoA,ROCK1 and ROCK2 were increased significantly in the hypoxia group(P<0.01).Compared with the hypoxia group,the mPAP and the relative mRNA expression levels of PCNA,CDK4,CyclinD1,RhoA,ROCK1 and ROCK2 in the lung tissues of the SPM group rats were all decreased significantly(P<0.05 or P<0.01).Conclusion:SPM can prevent the HAPH in rats,and the mechanisms may be related to the inhibition of the excessive proliferation of smooth muscle cells in pulmonary artery and the excessive activation of the RhoA/Rho kinase(ROCK)signaling pathway.

关 键 词：甘西鼠尾草 大鼠 高原 肺动脉高压 

分 类 号：R285.5[医药卫生—中药学]