纳米氧化锌经口染毒对C57BL/6J小鼠外周脏器的影响  

Effects of oral exposure to zinc oxide nanoparticles on the peripheral organs of C57BL/6J mice

作　　者：韩洁 田蕾 刘子怡 方振 袭著革 刘晓华 HAN Jie;TIAN Lei;LIU Zi-yi;FANG Zhen;XI Zhu-ge;LIU Xiao-hua(Tianjin Institute of Physical Education,Tianjin 301617;Institute of Environmental Medicine and Operational Medicine,Academy of Military Medical Sciences,Tianjin 300050;Binzhou Medical College,Yantai 264000,China)

机构地区：[1]天津体育学院,天津301617 [2]军事科学院军事医学研究院环境医学与作业医学研究所,天津300050 [3]滨州医学院,山东烟台264000

出　　处：《中国应用生理学杂志》2019年第6期555-559,I0002,共6页Chinese Journal of Applied Physiology

基　　金：军队重点项目(BWS17J025,ASW16J004,BEP16J001)。

摘　　要：目的:探讨纳米氧化锌经口染毒60 d对C57BL/6J小鼠多种外周脏器的损伤作用。方法:20只雄性C57BL/6J小鼠随机分为对照组和实验组,每组10只,实验组将纳米氧化锌溶液以20 mg/kg体重的剂量连续灌胃染毒60 d,对照组给予相应量的生理盐水;小鼠每周称重一次,染毒结束后,眼球取血,检测血糖、血脂、肝功能和肾功能相关指标,以及血清中炎症因子PAF、IL-6和TNF-α含量;取心脏、肝脏、脾脏、肺、肾脏和小肠组织制备病理切片,HE染色后,观察组织形态学变化。结果:实验组和对照组之间的体重无显著性差异;与正常对照组比较,实验组大鼠血中白蛋白(ALB)、白蛋白/球蛋白比值(A/G)、碱性磷酸酶(ALP)、谷草/谷丙转氨酶比值(S/L)、尿酸(UA)和尿素氮(BUN)含量明显升高(P<0.05或P<0.01);两组间血清中炎症因子含量无显著差异。病理学检查发现,实验组心肌中部分区域出现浊肿,肝脏出现轻度炎性病变(灶性或小灶性坏死),脾脏色素沉着减少,肺部出现轻或中度间质性炎症,肾脏和小肠未见明显病理改变。结论:纳米氧化锌经口染毒60 d未引起C57BL/6J小鼠血液系统炎症,但可诱导心脏、肝脏、脾脏和肺脏出现轻度的病理变化,并导致肝脏和肾脏的功能异常。Objective:To investigate the effects of oral exposure of zinc oxide nanoparticles on multiple peripheral organs of C57BL/6J mice.Methods:Twenty male C57BL/6J mice were randomly divided into control group and experimental group,with 10 mice in each group.The experimental group was treated with continuous gavage administration of zinc oxide nanoparticle solution at a dose of 20 mg/kg body weight for 60 days,and the control group was given the corresponding amount of normal saline;the mice were weighed once a week.After the end of the exposure,blood samples was collected from the eyeballs,and the levels of blood sugar and lipids,liver and kidney function,and inflammatory factors such as platelet activating factor(PAF),interleukin-6(IL-6)and tumor septicemia(TNF-α)were detected.Then,tissues sections of the heart,liver,spleen,lung,kidney and small intestine were prepared and their morphological changes were observed after hematoxylin-eosin staining.Results:There was no significant difference in body weight between control group and the experimental group.Compared with control group,the serum levels of albumin(ALB),albumin/globulin ratio(A/G),alkaline phosphatase(ALP)activity,aspartate aminotransferase/alanine aminotransferase ratio(AST/ALT),uric acid(UA)and blood urea in the experimental group were increased significantly(P<0.05 or P<0.01).There was no significant change in serum inflammatory factors.Pathological examination showed myocardial turbidity,mild inflammatory lesions(focal or small necrosis)in liver,decreased pigmentation in spleen,mild or moderate interstitial inflammation in lungs,and no obvious pathological changes in the kidneys or small intestine.Conclusion:Sixty days of oral exposure to nanometer zinc oxide did not cause inflammation in the blood system of C57BL/6J mice,but it could induce mild pathological changes in the heart,liver,spleen and lungs,and lead to abnormal liver and kidney function.

关 键 词：纳米氧化锌 小鼠 毒性 器官 

分 类 号：R114[医药卫生—卫生毒理学]