基于GenBank数据库的不同基因型细粒棘球绦虫系统进化分析  

Phylogenetic analysis of Echinococcus granulosus genotypes based on the GenBank database

作　　者：吴世乐[1] 朱文君 范得艳 石兵强 安永德[1] 王春亮 韩秀敏[1] 郭亚民[1] WU Shi-Le;ZHU Wen-Jun;FAN De-Yan;SHI Bing-Qiang;AN Yong-De;WANG Chun-Liang;HAN Xiu-Min;GUO Ya-Min(Qinghai Provincial Peopled Hospital,Qinghai Province^Xining 810007,China;Qinghai Provincial Red Cross Hospital,China)

机构地区：[1]青海省人民医院,西宁810007 [2]青海省红十字医院

出　　处：《中国血吸虫病防治杂志》2020年第1期36-46,59,共12页Chinese Journal of Schistosomiasis Control

基　　金：青海省卫生科技计划项目(2017-wjzdx-22)。

摘　　要：目的分析GenBank数据库中公布的细粒棘球绦虫各基因型细胞色素C氧化酶亚基Ⅰ(Cox1)基因序列,探索细粒棘球绦虫各基因型在全球不同地区的遗传变异及分化情况。方法收集GenBank数据库中公布的不同基因型细粒棘球绦虫Cox1基因序列,排除同一地区内相同基因序列,寻找收集的基因序列间的变异位点并构建进化树,观察不同地区细粒棘球绦虫各基因型的地域聚集性及原始基因序列。结果通过对Cox1基因序列变异位点统计,发现细粒棘球绦虫Cox1基因突变类型以颠换为主,G1、G6、G7基因型在其分布的各地区均有相同Cox1基因序列,基因序列相对应的GenBank登录号分别为KY766891、MH300971、MH301007。系统进化分析发现G10基因型存在明显地域聚集性。结论细粒棘球绦虫G1、G6、G7基因型存在原始Cox1基因序列;G10基因型进化树中出现地域聚集性,存在生殖隔离趋势。Objective To analyze the sequences of the cytochrome C oxidase subunit I(Cox1)gene of various Echinococcus granulosus genotypes that are currently recorded in the GenBank database,so as to investigate the genetic variation and differentiation of the E.granulosus genotypes across the world.Methods The sequences of the Cox1 gene of various E.granulosus genotypes that are currently recorded in the GenBank database were collected,and the same sequences of the Cox1 gene identified from a region were excluded.The mutation sites among the Cox1 gene sequences were identified and a phylogenetic tree was created based on the Cox1 gene.Results Transversion mutation was the predominant type of mutation in the Cox1 gene of E.granulosus.The same Cox1 gene sequence was found in E.granulosus G1,G6 and G7 genotypes isolated from various geographical locations across the world,with the corresponding GenBank accession numbers of KY766891,MH300971 and MH301007,respectively.Phylogenetic analysis revealed that E.granulosus G10 genotype had a remarkable geographical aggregation.Conclusions E.granulosus G1,G6 and G7 genotypes have primitive Cox1 gene sequences.There is a geographical aggregation of the E.granulosus G10 genotype in the phylogenetic tree,which has a tendency towards reproductive isolation.

关 键 词：细粒棘球绦虫 基因型 进化分析 细胞色素氧化酶亚基Ⅰ 

分 类 号：R383.33[医药卫生—医学寄生虫学]