三种不同饮食干预建立非酒精性脂肪肝模型  

Establishment of the Model of Nonalcoholic Fatty Liver Disease by Three Different Dietary Interventions

作　　者：张丹[1] 吕俊衍 邓溢 周波 耿坐涛 罗粟风 王金香 文玉[1] 应昀晏 文代艳 马岚青[1] ZHANG Dan;LV Jun-yan;DENG Yi;ZHOU Bo;GENG Zuo-tao;LUO Su-feng;WANG Jin-xing;WEN Yu;YING Yun-yan;WEN Dai-yan;MA Lan-qing(Dept.of Digestiver Medicine,The First Affiliated Hospital,Kunming Medical University,Kunming Yunnan 650032;Dept.of Clinical Medicine,Kunming Medical University,Kunming Yunnan 650500;Dept.of General Surgery,Lijiang Children's Hospital,Lijiang Yunnan 530721,China)

机构地区：[1]昆明医科大学第一附属医院消化内科,云南昆明650032 [2]昆明医科大学,云南昆明650500 [3]丽江市儿童医院普外科,云南丽江530721

出　　处：《昆明医科大学学报》2020年第1期11-17,共7页Journal of Kunming Medical University

基　　金：国家自然科学基金资助项目(81760108,81560099);云南省应用基础研究计划重点基金资助项目(2018FA039);云南省教育厅科学研究基金资助项目(2019Y0353);昆明医科大学研究生创新基金资助项目(2019S023);云南省科技厅-昆明医科大学应用基础研究联合专项基金资助项目[2018FE001(-027)]。

摘　　要：目的利用3种不同饮食干预建立小鼠非酒精性脂肪肝(NAFLD)模型,为NAFLD的机制研究提供参考依据。方法60只健康雄性C57BL/6小鼠,随机分为正常对照组(N)、高脂饮食组(HFD)、叶酸缺乏组(FD)和高同型半胱氨酸组(HCY),每组15只,造模14周。观察各组小鼠一般情况,测定胰岛素、空腹血糖及肝甘油三酯指标,并进行肝脏病理学检查。结果与N组比较,干预饮食14周后HFD组体重明显增加(P<0.0001),HCY组体重明显减轻(P<0.05);肝脏病理结果显示:HFD组、FD组及HCY组出现脂肪变性;HFD组胰岛素抵抗指数显著增高(P<0.01),各模型组的肝组织甘油三酯水平明显增高(P<0.05)。结论通过3种不同饮食干预成功诱导的NAFLD小鼠模型,方法简单,重复性强,可模拟人类不同病因引起的脂肪肝,为NAFLD的机制及药物治疗方面的研究提供理想的动物模型。Objective To establish a non-alcoholic fatty liver disease(NAFLD)research of model in mice by three different dietary interventions,and to provide a reference for the mechanism research of NAFLD.Methods Sixty healthy male C57BL/6 mice were randomly divided into normal control group(N),high-fat diet group(HFD),folic acid deficiency group(FD)and high-homocysteine group(HCY),15 in each group,and they were modeled for 14 weeks.The general situation of mice in groups were observed,the indexes of insulin and fasting glucose and hepatic triglyceride were measured,and the liver pathology was performed.Results Compared with the N group,the HFD group had a significant weight gain(P<0.0001)and the HCY group had a significant weight loss(P<0.05)after the intervention diet for 14 weeks.The liver pathological results showed that fatty degeneration appeared in the HFD group,FD group and HCY group.Insulin resistance index was significantly increased in the HFD group(P<0.01),Hepatic triglyceride level was significantly increased in each model group(P<0.05).Conclusion Three different dietary interventions can successfully induce NAFLD mouse model with simple and reproducible methods to simulate NAFLD with different etiologies,provide an ideal animal model for the study of the mechanism and drug therapy of NAFLD.

关 键 词：非酒精性脂肪肝 饮食 高脂肪 叶酸缺乏 高同型半胱氨酸 

分 类 号：R332[医药卫生—人体生理学]