高灵敏化学发光酶联免疫检测技术检测祛痘类化妆品中的氟喹诺酮类抗生素  

High-sensitive chemiluminescence immunoassay for determination of fluoroquinolones in cosmetics

作　　者：李启艳 于海英 胡德福 孙德军[2] 崔基炜 LI Qiyan;YU Haiying;HU Defu;SUN Dejun;CUI Jiwei(Shandong Institute for Food and Drug Control,Jinan 250101,China;School of Chemistry and Chemical Engineering,Shandong University,Jinan 250100,China)

机构地区：[1]山东省食品药品检验研究院,山东济南250101 [2]山东大学化学与化工学院,山东济南250100

出　　处：《药学研究》2020年第2期92-95,共4页Journal of Pharmaceutical Research

摘　　要：目的本研究建立了一种直接检测化妆品中氟喹诺酮类抗生素(FQs)的高特异性化学发光酶联免疫吸附试验(CL-ELISA)方法。方法辣根过氧化物酶(HRP)经高碘酸钠活化与抗氧氟沙星抗体进行偶联反应后,加入NaBH 4饱和甲醇溶液,充分进行还原反应后得到辣根过氧化物酶-氧氟沙星抗体标记物,采用直接竞争化学发光免疫法对抗体标记物进行检测。结果氟喹诺酮类检测限LOD(IC 10)为2.0 ng·mL-1;检测范围(IC 20~IC 80)为4.1~104.6 ng·mL-1;平均回收率范围在75.2%~98.2%,变异系数低于9.64%。交叉反应率结果表明该方法对恩诺沙星、环丙沙星、诺氟沙星、培氟沙星、洛美沙星、沙拉沙星等氟喹诺酮类抗生素有不同程度的交叉反应(12.5%~128.6%)。结论该方法简单、快速、准确度高,可用于化妆品中多种氟喹诺酮类抗生素高灵敏度快速检测。Objective This research presented a direct high-specific chemiluminescent enzyme-linked immunosorbent assay protocol(CL-ELISA)protocol for monitoring ofloxacin in cosmetics.Methods Horseradish peroxidase HRP was activated by sodium periodate and coupled with anti-ofloxacin antibody.After adding NaBH 4 saturated methanol solution,the HRP-ofloxacin antibody marker was obtained by full reduction reaction.The antibody marker was detected by direct competitive chemiluminescence immunoassay.Results The limit of detection(LOD)value,measured by IC 10,was 2.0 ng mL-1.The detection range,measured by IC 20~IC 80,was 4.1~104.6 ng mL-1.In spiked cosmetics samples,mean recoveries ranged from 75.2%to 98.2%,with variation less than 9.64%.This developed protocol showed different cross-reactivity value for fluoroquinolones tested(12.5%~128.6%).Conclusion The method was simple,rapid and accurate,and can be used for the rapid detection of fluoroquinolones in cosmetics with high specificity.

关 键 词：氟喹诺酮类抗生素 化学发光酶联免疫 直接免疫分析 化妆品 

分 类 号：R927.2[医药卫生—药学]