参苓白术散对非酒精性脂肪性肝病大鼠肝细胞、Kupffer细胞5-LO/LTB4通路的影响  

Effects of Shenling Baizhu Powder on 5-LO/LTB4 Pathway in Hepatocytes and Kupffer Cells of Nonalcoholic Fatty Liver Disease Rats

作　　者：徐拥建 冯高飞 张云城 黄裕华 邓远军[2] XU Yongjian;FENG Gaofei;ZHANG Yuncheng;HUANG Yuhua;DENG Yuanjun(Shenzhen Hospital(Longgang),Beijing University of Chinese Medicine,Shenzhen,Guangdong 518172,China;School of Traditional Chinese Medicine,Jinan University,Guangzhou,Guangdong 510632,China)

机构地区：[1]北京中医药大学深圳医院(龙岗),广东深圳518172 [2]暨南大学中医药学院,广东广州510632

出　　处：《湖南中医药大学学报》2020年第3期292-297,共6页Journal of Hunan University of Chinese Medicine

基　　金：北京中医药大学校级科研项目(2018BUCMXJKY013);深圳市龙岗区医疗卫生科技计划资助项目(20170405190544730)。

摘　　要：目的基于5-脂氧化酶(5-lipoxygenase,5-LO)/白三稀B4(leukotrienes B4,LTB4)通路探讨参苓白术散对非酒精性脂肪性肝病(nonalcoholic fatty liver disease,NAFLD)大鼠肝细胞、库普弗(Kupffer)细胞作用机制。方法通过Ⅳ型胶原酶离体循环灌注法分离SD大鼠肝细胞及Kupffer细胞,利用油红O染色观察肝组织脂滴蓄积情况;ELISA法检测肝组织LTB4含量;RT-PCR法、Western blot法检测大鼠肝细胞及Kupffer细胞5-LO、白三烯B4受体(LTB4 receptor type 1,BLT1)mRNA及蛋白表达水平。结果高脂饮食8周能诱导大鼠NAFLD表现,建模成功。与模型组比较,2个药物剂量干预组大鼠肝组织LTB4含量均显著下降(P<0.01);肝细胞、Kupffer细胞中的5-LO、BLT1 mRNA及其蛋白表达水平均亦有不同程度的下调(P<0.01)。2个剂量组间比较,以高剂量参苓白术散组效果较为显著(P<0.05或P<0.01)。结论参苓白术散可能通过抑制NAFLD大鼠肝细胞、Kupffer细胞5-LO/LTB4通路的激活,减轻肝脏脂质蓄积,发挥防治NAFLD的作用。Objective To investigate the action mechanism of Shenqi Baizhu Powder(SBP)on hepatocytes and Kupffer cells of nonalcoholic fatty liver disease(NAFLD)rats based on 5-lipoxygenase(5-LO)/leukotrienes B4(LTB4)pathways.Methods SD rat hepatocytes and Kupffer cells were isolated by in vitro perfusion of type IV collagenase.The hepatic lipid accumulation of liver tissue was detected by oil red O staining.The content of LTB4 in liver tissue were detected by enzyme-linked immunosorbent ELISA.The mRNA and proteins expression of 5-LO and LTB4 receptor type 1(BLT1)in were detected by RT-PCR and Western blot.Results It was observed that the rats of NAFLD model were induced by high-fat-diet with 8 weeks successfully.Compared with the model group,the content of LTB4 were decreased significantly in in the two drug intervention groups(P<0.01),as well as the lower expression levels of gene and proteins expression of 5-LO and BLT1 in hepatocytes and Kupffer cells for the varying degrees(P<0.01).Compared between two dose groups,the high-dose SBP group showed more significant effects(P<0.05 or P<0.01).Conclusion SBP may play a role in preventing and treating NAFLD by inhibiting the activation of 5-LO/LTB4 pathway in liver cells and Kupffer cells of NAFLD rats,and reducing liver lipid accumulation.

关 键 词：参苓白术散 肝细胞 KUPFFER细胞 5-脂氧化酶 白三稀B4 

分 类 号：R285.5[医药卫生—中药学]