肛门区鳞状细胞癌和鳞状上皮内瘤变组织中HPV感染型别的研究  

The Study of HPV infection types in tissues of squamous cell carcinoma and intraepithelial neoplasia of anus area

作　　者：季月霞 武晓雷 耿建祥[2] 仲玉英[1] 吴冲 吴昆岚[2] 张金浩[2] 梅静[2] 赵雪[2] JI Yuexia;WU Xiaolei;GENG Jianxiang;ZHONG Yuying;WU Chong;WU Kunlan;ZHANG Jinhao;MEI Jing;ZHAO Xue(Department of Pathology,the Traditional Chinese Medical Hospital of Changshu City,Changshu,Jiangsu 215500,China;Department of Pathology,the Affiliated Nanjing Traditional Chinese Medical Hospital of Nanjing Traditional Chinese Medical University,Nanjing,Jiangsu 210022,China)

机构地区：[1]南京中医药大学常熟附属医院病理科,江苏常熟215500 [2]南京中医药大学附属南京中医院病理科,江苏南京210022

出　　处：《国际检验医学杂志》2020年第6期652-655,共4页International Journal of Laboratory Medicine

基　　金：江苏省南京市卫生局中医专项(2009-92);江苏省常熟市中医院青年科研基金项目(cszyy201902)。

摘　　要：目的探讨肛门区鳞状细胞癌及肛门区上皮内瘤变(AIN)组织中人乳头瘤病毒(HPV)感染型别分布情况及临床意义。方法采用基因扩增结合基因芯片技术对28例肛门区鳞状细胞癌、20例低级别及34例高级别AIN组织标本进行23种HPV分型检测,并对受检者行相关资料分析。结果28例肛门区鳞状细胞癌总的HPV检出率为82.14%(23/28),检出的均为高危型HPV(HRHPV),占检出HPV的100.00%(23/23);20例低级别AIN中,HPV检出率为55.00%(11/20),HRHPV占检出HPV的45.46%(5/11),34例高级别AIN中,HPV检出率为79.41%(27/34),HRHPV占检出HPV的85.19%(23/27)。结论肛门区鳞状细胞癌和AIN的发生、发展与HRHPV感染密切相关,随着AIN程度的增加,HRHPV所占比例也随之增加。临床医师应对高危人群开展肛门区HPV分型检测及细胞学检查。Objective The anus squamous cell carcinoma(ASCCA)and anus intraepithelial neoplasm(AIN)are closely interrelated with human papillomavirus(HPV)infection.This is aimed to investigate the clinical value of the distribution of human papillomavirus(HPV)genotyping and its clinical significance in tissues of ASCCA and anus intraepithelial neoplasm(AIN).Methods PCR and and gene-chips technology were utilized for the detection of 23 kinds of HPV genetypes in tissue specimens from 28 cases of ASCCA and 20 cases of low-grad and 34 cases of high-grade AIN.And related materials of all subjects were analyzed.Results In the 28 cases of ASCCA,the HPV detection rate was 82.14%(23/28)and high-risk human papillomavirus(HRHPV)counted for 100.00%(23/23).In the 20 cases of low-grade AIN,the HPV detection rate was 55.00%(11/20)and HRHPV counted for 45.46%(5/11).In the 34 cases of high-grade AIN,the HPV detection rate was 79.41%(27/34)and HRHPV counted for 85.19%(23/27).Conclusion The occurrence of ASCCA and AIN are related to HRHPV infection,the HRHPVs gradually increase with aggravation of anal squamous epithelial lesions.Clinical doctors should conduct anal HPV type tests and cytological examination on high-risk affected population.

关 键 词：肛门 人乳头瘤病毒 鳞状细胞癌 上皮内瘤变 基因分型 

分 类 号：R711.74[医药卫生—妇产科学]