甘草提取物-甘草素HPLC-DAD含量测定方法研究  

Research on the Determination Method of Glycyrrhizin in Licorice Extract

作　　者：秦昆明 陈海超 伏东宁 李祥永[3] 常志惠 周越[3] 李轩涛 金俊杰 王卿[4] QIN Kun-ming;CHEN Hai-chao;FU Dong-ning;LI Xiang-yong;CHANG Zhi-hui;ZHOU Yue;LI Xuan-tao;JIN Jun-jie;WANG Qing(Nanjing Haichang Chinese Medicine Corporation,Nanjing 210061,China;Nanjing Haiyuan Prepared Slices of Chinese Crude Drugs Co.Ltd,Nanjing 210061,China;Lianyungang Traditional Chinese Medicine Hospital,Jiangsu,Lianyungang 222004;Wuxi Traditional Chinese Medicine Hospital,Jiangsu,Wuxi 214001,China)

机构地区：[1]南京海昌中药集团有限公司,江苏南京210061 [2]南京海源中药饮片有限公司,江苏南京210061 [3]连云港市中医院,江苏连云港222004 [4]无锡市中医医院,江苏无锡214001

出　　处：《海峡药学》2020年第2期30-32,共3页Strait Pharmaceutical Journal

基　　金：“重大新药创制”科技重大专项(2017ZX09301075);江苏省中医药局科技项目资助(PY201518);江苏省科技成果转化专项资金项目(BA2018002);江苏省六大人才高峰项目(SWYY-108);江苏省333高层次人才工程项目(2016-3-0277);南京市计划项目(201812021)。

摘　　要：目的建立甘草提取物甘草素的含量测定方法。方法采用HPLC法对甘草提取物中的主要成分甘草素进行定量分析。色谱柱为YMC-Pack ODS-A C18柱(4.6mm×250mm,5μm);以0.1%磷酸水(A)-乙腈(B)为流动相,等度洗脱,流速1mL·min^-1,柱温30℃,进样量10μL,检测波长280nm。结果甘草素在5.02~104μg·mL^-1浓度范围内呈现良好的线性关系,y=28944X-132953,R2=0.9997,平均回收率为99.99%,RSD为0.23%。结论本法准确可靠、专属性强,结果稳定,重复性好,可用于甘草提取物中甘草素的含量测定。OBJECTIVE To establish a method for determining the content of licorice extract glycyrrhizin.METHODS The main component of glycyrrhizin in licorice extract was quantitatively analyzed by HPLC.HPLC column was YMC-Pack ODS-A C18(4.6 mm×250 mm,5μm).The mobile phase was 0.1% phosphoric acid water(A)-acetonitrile(B),and isocratic elution with the flow rate of 1.0 mL·min^-1.The sample size was 10μL,and column temperature was 30℃.The detection wavelength was 280 nm.RESULTS Glycyrrhizin was linear in the range of 5.02~104μg·mL^-1,y=28944 X-132953,R2=0.9997.The average recovery was 99.99%,RSD was 0.23%.CONCLUSION The method is accurate,reliable and specific.The results are stable with good repeatability.It can be usead for the determine of of licorice extract glycyrrhizin.

关 键 词：甘草提取物 甘草素 HPLC-DAD 含量测定 

分 类 号：R284[医药卫生—中药学]