锆离子膜修饰电极生物传感器的制备及其电化学性能研究  

A Study of the Preparation and Electrochemical Properties of Biosensor with Zirconium Ion Membrane Modified Electrode

作　　者：任勇[1] 张勐[2] 刘钊 Ren Yong;Zhang Meng;Liu Zhao

机构地区：[1]淄博师范高等专科学校宣传统战处,山东淄博255130 [2]淄博师范高等专科学校办公室,山东淄博255130

出　　处：《淄博师专论丛》2020年第1期67-70,共4页Forum of Zibo Normal College

摘　　要：在富含羧基的碳糊电极表面自组装锆离子膜,通过锆离子膜与DNA磷酸骨架的静电吸附作用制得电化学生物传感器,并用循环伏安法对锆离子膜修饰电极进行了电化学表征。研究发现,锆离子膜极大地改变了电极的表面性质。以亚甲蓝(MB)为杂交指示剂用循环伏安法优化了锆离子膜修饰电极的制备条件,并用微分脉冲伏安法对NPT II特定基因序列进行了定性检测,效果良好。The electrochemical biosensor is prepared by self-assembly of zirconium ion film on the surface of carboxyl rich carbon paste electrode and electrostatic adsorption of zirconium ion film and DNA phosphate skeleton.The electrochemical characterization of zirconium ion film modified electrode iss carried out by cyclic voltammetry.It is found that the surface properties of the electrode are greatly changed by the zirconium film.The preparation conditions of zirconium ion film modified electrode are optimized by cyclic voltammetry with methylene blue(MB)as hybridization indicator,and the specific gene sequence of NPT II is qualitatively detected by differential pulse voltammetry.

关 键 词：修饰电极 锆离子膜 微分脉冲伏安法 NPT II基因 

分 类 号：TB34[一般工业技术—材料科学与工程]