四倍体楸叶泡桐体外植株再生系统建立  

Establishment of the System of in Vitro Plantlet Regeneration of Autotetraploid Paulownia catalpifolia Plant

作　　者：张变莉[1] 王杨[2] 刘荣宁[1] 范国强[2] Zhang Bianli;Wang Yang;Liu Rongning;Fan Guoqiang(Henan Vocational College of Agriculture,Zhongmu 451450,China;Institute of Paulownia,Henan Agricultural University,Zhengzhou 450002,China)

机构地区：[1]河南农业职业学院,河南中牟451450 [2]河南农业大学泡桐研究所,河南郑州450002

出　　处：《河南林业科技》2019年第4期4-7,47,共5页Journal of Henan Forestry Science and Technology

基　　金：河南省郑州市普通科技攻关项目(153PKJGG423);河南省杰出人才创新基金项目(321001700);河南省高校杰出科研人才工程基金项目(2002KYC-003)。

摘　　要：将楸叶泡桐四倍体的叶片和叶柄分别置于含有不同质量浓度的6-BA(6~20 mg/L)和NAA(0.1~1.1 mg/L)的组合培养基上诱导再生植株,筛选最佳外植体与最适培养基,建立同源四倍体楸叶泡桐体外植株再生系统。结果表明,同源四倍体楸叶泡桐体外植株再生的最佳外植体是叶片;其幼苗叶片愈伤组织诱导的最适培养基为MS+14 mg/L 6-BA+0.3 mg/L NAA;愈伤组织芽诱导的最适培养基为MS+16 mg/L 6-BA+0.3 mg/L NAA;幼芽生根的最适培养基为1/2MS。The system of in vitro plantlet regeneration from the leaves of autotetraploid of Paulownia catalpifolia was established in order to lay foundation for the breeding of new variant of Paulownia plants.The induction of in vitro plantlet regeneration from the leaves and peioles of autotetraploid with different hormones and concentrations(0.1-1.1 mg/L NAA,6-20 mg/L 6-BA)of media were investigated in order to screen the optimal explants and media.The result indicated that the leaves were the optimal explants for in vitro plantlet regeneration of autotetraploid Paulownia catalpifolia plant.Moreover,the optimal media of callus induction from Paulownia catalpifolia was MS+0.3 mg/L NAA+14 mg/L 6-BA,and that MS+0.3 mg/L NAA+16 mg/L 6-BA might be optimal its shoot induction media;and the optimal rooting media of the shoot was 1/2MS media.

关 键 词：楸叶泡桐 同源四倍体 体外植株再生 

分 类 号：S792.43[农业科学—林木遗传育种]