低平均光强下波动光对铜绿微囊藻生长的影响  

EFFECTS OF FLUCTUATING LIGHT ON THE GROWTH OF MICROCYSTIS AERUGINOSA UNDER LOW LIGHT AVERAGE INTENSITY

作　　者：孙昕[1] 汤加刚 李鹏飞[1] 孙杰[1] 何飞飞 由黎 贺健程 SUN Xin;TANG Jia-Gang;LI Peng-Fei;SUN Jie;HE Fei-Fei;YOU Li;HE Jian-Cheng(Key Laboratory of Environmental Engineering,Shaanxi Province,Key Laboratory of Northwest Water Resources,Environment and Ecology,Ministry of Education,Xi’an University of Architecture and Technology,Xi’an 710055 China)

机构地区：[1]西安建筑科技大学陕西省环境工程重点实验室,西北水资源与环境生态教育部重点实验室,西安710055

出　　处：《水生生物学报》2020年第2期438-444,共7页Acta Hydrobiologica Sinica

基　　金：国家自然科学基金(51178379和51278404);陕西省科技统筹创新工程计划项目(2015KTCL-03-15)资助.

摘　　要：为了研究波动光对藻类的影响,以典型水华藻种铜绿微囊藻Microcystis aeruginos为研究对象,运用了基于单片机系统的光强控制实验装置,开展了不同光照条件下铜绿微囊藻的生长研究。共设置了四种光照条件,分别为不同周期波动光强FL(Fluctuating Light)组(10min FL、1h FL和6h FL)和平均光强AL(Average Light)组。实验结果表明,在低平均光强下,6h FL、1h FL和10min FL组铜绿微囊藻藻密度相对于AL组分别增加了28.3%(P<0.05)、18.2%(P<0.05)和7.7(P>0.05)。三组波动光强下铜绿微囊藻的比增长速率、Fv/Fm和rETR均显著大于平均光强组(P<0.05),且随着波动光周期的增大,各指标也会显著增加(P<0.05),而热耗散NPQ平均值、单个细胞类胡萝卜素含量等指标与上述指标呈相反的规律并且差异显著(P<0.05)。结果也表明在低平均光强下,相比于恒定光照,铜绿微囊藻在波动光下能更好地调节自身光合作用机制去利用光能,且波动周期越大,铜绿微囊藻对光能利用效率越高。这暗示了低强度波动光可以作为提高藻类产量的一种手段。To study the effects of fluctuating light on algae,the growth of Microcystis aeruginosa,a typical water bloom alga,was studied under different light conditions using an experimental device with a light intensity control system based on a single-chip system.Four light conditions were applied in this research:three fluctuating light groups(10min FL,1h FL and 6h FL)with different fluctuation cycles and an average light group(AL).The experimental results show that the cell densities of Microcystis aeruginosa in the 6h FL,1h FL and 10min FL groups were 28.3%(P<0.05),18.2%(P<0.05)and 7.7(P>0.05),respectively,which were higher than that in the AL group.It was also found that the specific growth rate,Fv/Fm and rETR of Microcystis aeruginosa in the three fluctuating light groups were significantly higher than those in the AL group(P<0.05),and each index significantly increased with the length of the fluctuating light cycle,but the mean NPQ and carotenoids per unit dry weight showed the opposite relationship to fluctuating light.The results also showed that Microcystis aeruginosa could better regulate their photosynthesis mechanism to utilize light energy under fluctuating light than under constant light when the average light intensity was low,and the longer the fluctuating period was,the better the utilization efficiency.This implies that fluctuating low-intensity light can be used as a means to increase algae production.

关 键 词：波动光 周期 叶绿素荧光参数 平均光强 铜绿微囊藻 

分 类 号：Q948.8[生物学—植物学]