Screening and degradation characteristics of a tylosin-degrading strain  

作　　者：FENG Chang-qing CHENG Deng-miao FENG Yao QI Wei-ning JIA Zhen-hu Louise WEAVER LIU Yuan-wang LI Zhao-jun 

机构地区：[1]College of Life Science,Shanxi Normal University,Linfen 041004,P.R.China [2]Key Laboratory of Plant Nutrition and Fertilizer,Ministry of Agriculture and Rural Affairs/China-New Zealand Joint Laboratory for Soil Molecular Ecology,Institute of Agricultural Resources and Regional Planning,Chinese Academy of Agricultural Sciences,Beijing 100081,P.R.China [3]Institute of Environmental and Science Research Ltd.,Christchurch Science Centre,Christchurch 8041,New Zealand

出　　处：《Journal of Integrative Agriculture》2020年第4期1127-1136,共10页农业科学学报（英文版）

基　　金：jointly supported by the National Key R&D Program of China(2018YFD0500206);the National Natural Science Foundation of China(31772395);the Fundamental Research Funds for Central Non-profit Scientific Institution,Chinese Academy of Agricultural Sciences(1610132019046)。

摘　　要：Antibiotics residues have been accumulating in the environment day by day due to overuse of antibiotics.Recalcitrant antibiotic residues,such as tylosin(TYL),can cause serious environmental problems,which makes it important to eliminate TYL from the environment.It is important to eliminate TYL from the environment.In this study,a strain was isolated and purified from fermentation by-product that came from a TYL production factory.The TYL degrading strain was identified by its morphology,physiological and biochemical reactions and sequencing the PCR-amplified fragments of its 16 S r DNAcoding genes.The temperature,shaking speed,initial TYL concentration,p H and inoculum sizes were investigated under simulated conditions by using single factor tests.The results showed that TYL2,a high efficient strain was isolated and was identified as Brevibacillus borstelensis.The degradation rate of TYL by this strain could reach to 75%with an initial concentration of 25 mg L^-1 within 7 days under conditions of 7%B.borstelensis(v/v,2×108 CFU m L^-1)at p H 7.0 and at 35°C.It is interesting that this strain has a very strong ability to degrade the TYL in natural sewage with the degradation rate of 65%within 7 days.This result could be helpful for the degradation of TYL and provide guidance for the degradation of other antibiotics.Antibiotics residues have been accumulating in the environment day by day due to overuse of antibiotics. Recalcitrant antibiotic residues, such as tylosin（TYL）, can cause serious environmental problems, which makes it important to eliminate TYL from the environment. It is important to eliminate TYL from the environment. In this study, a strain was isolated and purified from fermentation by-product that came from a TYL production factory. The TYL degrading strain was identified by its morphology, physiological and biochemical reactions and sequencing the PCR-amplified fragments of its 16 S r DNAcoding genes. The temperature, shaking speed, initial TYL concentration, p H and inoculum sizes were investigated under simulated conditions by using single factor tests. The results showed that TYL2, a high efficient strain was isolated and was identified as Brevibacillus borstelensis. The degradation rate of TYL by this strain could reach to 75% with an initial concentration of 25 mg L–1 within 7 days under conditions of 7% B. borstelensis(v/v, 2×108 CFU m L–1) at p H 7.0 and at 35°C. It is interesting that this strain has a very strong ability to degrade the TYL in natural sewage with the degradation rate of 65% within 7 days. This result could be helpful for the degradation of TYL and provide guidance for the degradation of other antibiotics.

关 键 词：ANTIBIOTICS TYLOSIN SCREENING degrading STRAIN B.borstelensis DEGRADATION CHARACTERISTIC 

分 类 号：X703[环境科学与工程—环境工程] X172