Genomic landscape and genetic manipulation of the black soldier fly Hermetia illucens,a natural waste recycler  

作　　者：Shuai Zhan Gangqi Fang Minmin Cai Zongqing Kou Jun Xu Yanghui Cao Liang Bai Yixiang Zhang Yongmao Jiang Xingyu Luo Jian Xu Xia Xu Longyu Zheng Ziniu Yu Hong Yang Zhijian Zhang Sibao Wang Jeffery K.Tomberlin Jibin Zhang Yongping Huang 

机构地区：[1]CAS Key Laboratory of Insect Developmental and Evolutionary Biology,CAS Center for Excellence in Molecular Plant Sciences,Institute of Plant Physiology and Ecology,Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences,Shanghai 200032,China [2]CAS Center for Excellence in Biotic Interactions,University of Chinese Academy of Sciences,Beijing 100049,China [3]State Key Laboratory of Agricultural Microbiology,National Engineering Research Center of Microbial Pesticides,College of Life Science and Technology,Huazhong Agricultural University,Wuhan,Hubei 430070,China [4]Hubei Key Laboratory of Genetic Regulation and Integrative Biology,Institute of Entomology,School of Life Sciences,Central China Normal University,Wuhan,Hubei 430079,China [5]School of Economics,Zhejiang Gongshang University,Hangzhou,Zhejiang 310018,China [6]Department of Entomology,Texas A&M University,2475 TAMU,College Station,TX 77845,USA

出　　处：《Cell Research》2020年第1期50-60,共11页细胞研究（英文版）

基　　金：This study was supported by the Chinese Academy of Sciences(XDB11010600,KFZD-SW-219,QYZDB-SSW-SMC029 and XDB27040205);the National Key Technology R&D Program of China(2018YFD0500203).

摘　　要：The black soldier fly(BSF),Hermetia illucens(Diptera:Stratiomyidae),is renowned for its bioconversion of organic waste into a sustainable source of animal feed.We report a high-quality genome of 1.1 Gb and a consensus set of 16,770 gene models for this beneficial species.Compared to those of other dipteran species,the BSF genome has undergone a substantial expansion in functional modules related to septic adaptation,including immune system factors,olfactory receptors,and cytochrome P450s.We further profiled midgut transcriptomes and associated microbiomes of BSF larvae fed with representative types of organic waste.We find that the pathways related to digestive system and fighting infection are commonly enriched and that Firmicutes bacteria dominate the microbial community in BSF across all diets.To extend its potential practical applications,we further developed an efficient CRISPR/Cas9-based gene editing approach and implemented this to yield flightless and enhanced feeding capacity phenotypes,both of which could expand BSF production capabilities.Our study provides valuable genomic and technical resources for optimizing BSF lines for industrialization.

关 键 词：BLACK LANDSCAPE MANIPULATION 

分 类 号：Q78[生物学—分子生物学]