迟缓爱德华菌外膜蛋白TolC原核表达及其免疫原性  

Prokaryotic expression and immunological protection analysis of TolC protein of Edwardsiella tarda

作　　者：张志强[1] 杨楠[1] 李永慧 苏硕青 吴同垒[1] 康元环[3] 王洪彬 朱国强[4] 史秋梅[1] ZHANG Zhi-qiang;YANG Nan;LI Yong-hui;SU Shuo-qing;WU Tong-lei;KANG Yuan-huan;WANG Hong-bin;ZHU Guo-qiang;SHI Qiu-mei(Hebei Key Laboratory of Preventive Veterinary Medicine,Hebei Normal University of Science&Technology,Qinhuangdao,Hebei 066004,China;The Second Hospital of Qinhuangdao,Qinhuangdao,Hebei 066604,China;College of Animal Science and Technology,Jilin Agricultural University,Changchun 130118,China;College of Veterinary Medicine,Yangzhou University,Yangzhou,Jiangsu 225009,China)

机构地区：[1]河北科技师范学院河北省预防兽医学重点实验室,河北秦皇岛066004 [2]秦皇岛第二医院,河北秦皇岛066604 [3]吉林农业大学动物科学技术学院,吉林长春130118 [4]扬州大学兽医学院,江苏扬州225009

出　　处：《中国兽医学报》2020年第2期339-344,共6页Chinese Journal of Veterinary Science

基　　金：国家重点研发计划基金资助项目(2017YFD0501000);中国博士后科学基金资助项目(2017M611935);河北省科技厅奖励性后补助基金资助项目(15926620H);秦皇岛市科技局科技支撑计划资助项目(201602A341);河北科技师范学院博士启动基金资助项目(2015YB002)。

摘　　要：TolC是革兰阴性细菌一种外膜通道蛋白,参与细菌应对外界不利环境的耐受,沙门菌TolC蛋白作为免疫原对动物沙门菌感染的保护效果优良,具有潜在的应用价值。目前,迟缓爱德华菌(Edwardsiella tarda,E.tarda)该蛋白的研究尚未见有报道,本研究以E.tarda TolC蛋白作为研究对象,原核表达并纯化TolC蛋白,进一步通过动物试验确定该蛋白具有较强的免疫原性,免疫动物对致病性E.tarda ET-13强毒株感染具有较高的抵抗力。结果表明,E.tarda TolC蛋白具有较强的免疫原性和潜在的疫苗价值,为进一步研制E.tarda亚单位疫苗提供理论资料。TolC,a kind of outer membrane pathway protein of Gram-negative bacteria,play crucial roles in bacterial stress and drug resistance.This protein,as an immunogen,displays excellent immunogenicity and considerable protection in Sallmoella caused infection,suggesting its potential value as vaccine candidate.The TolC in Edwardsiella tarda is not elucidate well,and in this study,this was selected for prokaryotic expression and further purification.The recombinant TolC were performed as immunogen for further immunogenicity and protective immunity analysis.The results showed that the TolC protein is able to stimulate strong immune response and perfect specific protection to E.tarda infection.All these work provided guidance and foundation for development of subunit vaccine of E.tarda.

关 键 词：E.tarda 外膜通道蛋白 TOLC 蛋白表达 免疫保护力 

分 类 号：S852.61[农业科学—基础兽医学] R535[农业科学—兽医学]