菊苣酸(CA)对LPS诱导的牦牛PBMC细胞炎性因子分泌及表达的影响  

Effects of chicory acid on the secretion and expression of inflammatory factors by LPS-induced PBMC cells in yak

作　　者：阿顺贤 张元新 王爱超 张文颖 武强 薛才华 吴华[1] A Shun-xian;ZHANG Yuan-xin;WANG Ai-chao;ZHANG Wen-ying;WU Qiang;XUE Cai-hua;WU Hua(College of Agriculture and Animal Husbandry,Qinghai University,Xining 810016,China;Qinghai Datong Cattle Farm,Xining 810102,China)

机构地区：[1]青海大学农牧学院,青海西宁810016 [2]青海大通种牛场,青海西宁810102

出　　处：《中国兽医学报》2020年第2期379-385,共7页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目(31260567)。

摘　　要：为了探究菊苣酸(chioric acid,CA)对脂多糖(lipopolysaccharide,LPS)诱导的牦牛外周血单个核细胞(peripheral blood mononuclear cell,PBMC)炎性相关因子分泌及转录水平的调节作用,用Ficoll法分离牦牛PBMC,体外将PBMC与LPS和CA共同培养,通过CCK-8法筛选LPS最佳刺激浓度,ELISA试剂盒检测CA对炎性相关因子含量的影响,实时荧光定量PCR法检测炎性相关因子mRNA表达情况。结果表明,CCK-8法筛选的LPS最佳致炎质量浓度为1.0 mg/L。与对照组相比,CA处理组显著提高IL-10的含量(P<0.05),LPS处理组显著提高IL-6和IL-1β的含量(P<0.05),极显著提高了IL-8、TNF-a和INF-γ的含量(P<0.01);与LPS处理组相比,CA+LPS处理组IL-6、IL-8、IL-1β、TNF-a和INF-γ的含量均显著降低(P<0.05),但IL-10的含量显著升高(P<0.05)。同时,与对照组相比,CA处理组显著降低IL-1βmRNA表达(P<0.05),显著升高IL-10 mRNA表达(P<0.05),LPS处理组极显著升高IL-6、IL-8、IL-1β、INF-γ和TNF-αmRNA表达(P<0.01);与LPS组相比,CA+LPS处理组IL-6、INF-γ和TNF-αmRNA表达显著降低(P<0.05),IL-8和IL-1βmRNA表达极显著降低(P<0.01),IL-10 mRNA表达显著升高(P<0.05)。上述结果说明,CA可通过调节牦牛PBMC炎性因子的分泌及表达,从而发挥抗炎作用。In order to investigate the regulation of chicoric acid(CA) on lipopolysaccharide(LPS)-induced secretion and transcriptional levels of inflammatory related factors in peripheral blood mononuclear cells(PBMC) of yak.The PBMC of yak was isolated by Ficoll method,co-cultured the PBMC with LPS and CA in vitro,using CCK-8 to ascertain the optimal stimulation concentration of LPS;the effect of CA on the content of inflammatory related factors was detected by ELISA kit;the expression of inflammatory related factor was detected by real-time quantitative PCR.The results showed that the optimal concentration of LPS was 1.0 mg/L.Compared with the control group,the CA treatment group significantly increased the contents of IL-10(P<0.05),the LPS treatment group significantly increased the contents of IL-6 and IL-1β(P<0.05),and extremely significant increasing the contents of IL-8,TNF-a and INF-γ(P<0.01).Compared with the LPS treatment group,the levels of IL-6,IL-8,IL-1β,TNF-a and INF-γ were significantly decreased in the CA+LPS treatment group(P<0.05),but the contents of IL-10 was significantly increased(P<0.05).At the same time,compared with the control group,the CA treatment group significantly decreased the mRNA expression of IL-1β(P<0.05),significantly increased the mRNA expression of IL-10(P<0.05),and the LPS treatment group extremely significant increased the mRNA expression of IL-6,IL-8,IL-1β,INF-γand TNF-α (P <0.01);Compared with LPS treatment group,the mRNA expression of IL-6,INF-γand TNF-αwas significantly decreased in CA+LPS treatment group(P<0.05),and the mRNA expression of IL-8 and IL-1βwas extremely significant decreased(P<0.01),the mRNA expression of IL-10 was significant increased(P<0.05).The above results indicated that cichoric acid can exert an anti-inflammatory effect by regulating the secretion and expression of inflammatory factors in PBMC of yak.

关 键 词：菊苣酸(CA) LPS 牦牛 外周血单个核细胞 炎性因子 

分 类 号：S859.7[农业科学—临床兽医学] S852.4[农业科学—兽医学]