慢性乙型肝炎患者STING表达与病毒载量及肝组织病理的相关性  

Correlation between expression of STING and viral load and liver histopathology of patients with chronic hepatitis B

作　　者：王煜[1] 马娟[1] 张旭[1] 张爱芸[1] 盛慧萍[1] 陆晓莉 WANG Yu;MA Juan;ZHANG Xu;ZHANG Ai-yun;SHENG Hui-ping;LU Xiao-li(General Hospital of Ningxia Medical University,Yinchuan,Ningxia 750004,China)

机构地区：[1]宁夏医科大学总医院感染疾病科,宁夏银川750004 [2]宁夏回族自治区妇幼保健院保健部,宁夏银川750004

出　　处：《中华医院感染学杂志》2020年第1期82-85,共4页Chinese Journal of Nosocomiology

基　　金：宁夏回族自治区卫生计生系统重点基金资助项目(2016-NW-060)。

摘　　要：目的分析干扰素刺激基因(STING)在慢性乙型肝炎患者(CHB)中的表达情况,并探讨其与乙型肝炎病毒(hepatitis B virus,HBV) DNA载量、肝组织病理学的关系。方法选取108例慢性乙型肝炎患者(CHB组)和60例健康体检自愿者(对照组)作为研究对象,采用实时荧光定量PCR检测STING mRNA表达和HBV DNA载量、采用酶联免疫吸附法检测干扰素-α(interferon-α,IFN-α)和干扰素-β(interferon-α,IFN-β)表达水平,根据肝穿刺活检结果及病理学分级标准对CHB进行肝组织炎症分级和纤维化分级,并分析上述指标之间的关系。结果 CHB组STING mRNA(8.25±1.08)、INF-α(38.46±3.04)IU/ml、INF-β(35.17±2.86)IU/ml较对照组明显降低(P<0.05)。根据HBV DNA载量将CHB组分为四组,四组血清STING mRNA比较差异有统计学意义(F=26.492,P<0.001)。CHB组中不同肝组织炎症程度和纤维化程度患者STING mRNA表达差异明显(P<0.05)。经Pearson法分析,STING mRNA与INF-α、INF-β均呈正相关(P<0.05)。结论 STING在慢性乙型肝炎中低表达,并伴IFN-α/β明显降低,与患者HBV DNA载量、肝组织炎症活动度及纤维化程度相关。OBJECTIVE To investigate the expression of stimulator of interferon genes(STING) in the patients with chronic hepatitis B(CHB) and explore the its relationship with the hepatitis B virus(HBV) DNA load and liver histopathology. METHODS A total of 108 patients with CHB(the CHB group) and 60 people who volunteered for physical examination(the control group) were recruited as the study objects.The expression of STING mRNA and HBV DNA load were detected by using real-time fluorescence quantitative PCR, the levels of interferon-α(IFN-α) and interferon-β(IFN-β) were determined with the use of enzyme linked immunosorbent assay, the liver inflammation and fibrosis were graded for the CHB patients based on the results of liver biopsy and pathological grading criteria, and the relationship between the above indexes was observed. RESULTS The levels of STING mRNA, INF-α and INF-β of the CHB group were respectively(8.25±1.08),(38.46±3.04)IU/ml and(35.17±2.86)IU/ml, significantly lower than those of the control group(P<0.05).The patients of the CHB group were divided into four groups according to the HBV DNA load, and there was significant difference in the serum STING mRNA among the four groups(F=26.492,P<0.001).There was significant difference in the expression of STING mRNA among the patients with different grades of liver inflammation and fibrosis in the CHB group(P<0.05).Pearson analysis showed that STING mRNA was positively correlated with INF-α and INF-β(P<0.05). CONCLUSION The STING shows a low expression in the CHB patients, the IFN-α/β is remarkably declined, which is associated with the HBV DNA load, the degree of inflammation and fibrosis in liver tissues.

关 键 词：慢性乙型肝炎 干扰素刺激基因 DNA载量 肝组织病理 

分 类 号：R512.62[医药卫生—内科学]