热敏灸“中脘”穴对慢性萎缩性胃炎模型大鼠血清GH、PG的影响  

Effect of heat-sensitive moxibustion at "Zhongwan"(CV 12) on serum GH and PG in chronic atrophic gastritis rats

作　　者：章海凤[1] 罗淑瑜 张瑶 胡兵 李琳慧 张波[1] 葛来安[1] 康明非[1] 付勇[1] 熊俊[1] ZHANG Hai-feng;LUO Shu-yu;ZHANG Yao;HU Bing;LI Lin-hui;ZHANG Bo;GE Lai-an;KANG Ming-fei;FU Yong;XIONG Jun(department of Acupuncture and Moxibustion,Affiliated Hospital of Jiangxi University of TCM,Nanchang 330006,China;Graduate School of Jiangxi University of TCM)

机构地区：[1]江西中医药大学附属医院针灸科,南昌330006 [2]江西中医药大学研究生院

出　　处：《中国针灸》2020年第3期279-284,共6页Chinese Acupuncture & Moxibustion

基　　金：国家自然科学基金资助项目:81560793;江西省卫计委中医科研课题项目:2018A025。

摘　　要：目的:观察热敏灸"中脘"穴对慢性萎缩性胃炎(CAG)模型大鼠血清生长激素(GH)、胃蛋白酶原(PG)的影响,探讨热敏灸治疗CAG的可能作用机制。方法:将66只雄性SD大鼠随机分为空白组(12只)和造模组(54只)。空白组不予干预;造模组采用复合病因造模法造模12周后随机分为模型组(11只)、维酶素组(11只)、艾灸组(22只)。艾灸组悬灸"中脘"穴40 min,艾灸后按2 mL·kg-1·d-1灌服0.9%氯化钠溶液,根据悬灸过程中大鼠尾温变化分为热敏灸组(11只)和非热敏灸组(8只);维酶素组等量灌服维酶素溶液。每日1次,共28 d。观察各组大鼠体质量的变化;ELISA法检测血清GH、PGⅠ、PGⅡ表达,并计算PGⅠ/PGⅡ值(PGR);肉眼、光镜下观察胃黏膜形态学变化。结果:①造模后,造模组大鼠体质量低于空白组(P<0.01)。干预后,与模型组比较,维酶素组、非热敏灸组和热敏灸组大鼠体质量均明显增长(P<0.05);各干预组组间比较差异无统计学意义(P>0.05)。②造模组大鼠肉眼及光镜下胃黏膜色暗红或灰白,皱襞变平,充血明显,固有层腺体萎缩甚至消失,且排列稀疏不规则,可见淋巴滤泡和炎性细胞。干预后,维酶素组、非热敏灸组和热敏灸组胃黏膜形态均改善。③与空白组比较,模型组大鼠血清GH、PGⅠ、PGⅡ水平及PGR均明显降低(P<0.05,P<0.01);与模型组比较,维酶素组、非热敏灸组、热敏灸组大鼠血清GH、PGⅠ、PGⅡ水平均明显升高(P<0.05,P<0.01),PGR升高但差异无统计学意义(P>0.05);热敏灸组血清GH、PGⅠ水平高于非热敏灸组及维酶素组(P<0.05)。结论:热敏灸"中脘"穴可能通过上调血清GH、PGⅠ表达改善CAG大鼠胃黏膜形态。Objective To observe the effect of heat-sensitive moxibustion at "Zhongwan"(CV 12) on serum growth hormone(GH) and pepsinogen(PG) in chronic atrophic gastritis(CAG) rats, and to explore the potential mechanism of heat-sensitive moxibustion for CAG. Methods A total of 66 male SD rats were randomized into a blank group(12 rats) and a model establishment group(54 rats). No intervention was given in the blank group. Rats in the model establishment group were intervented with compound pathogeny method for 12 weeks to establish CAG model, which were further divided into a model group(11 rats), a vitacoenzyme group(11 rats) and a moxibustion group(22 rats). In the moxibustion group, suspending moxibustion was applied at "Zhongwan"(CV 12) for 40 min. After the intervention of moxibustion, 0.9% sodium chloride solution was given by gavage(2 mL·kg-1·d-1). According to the changes of tail temperature, rats in the moxibustion group were divided into a heat-sensitive moxibustion group(11 rats) and a non-heat-sensitive moxibustion group(8 rats). The vitacoenzyme group was given vitacoenzyme as the same dose by gavage. The intervention was adopted once a day for 28 days. Changes of body weight were observed among the groups. Expressions of serum GH, PGⅠand PGⅡwere detected by ELISA, and the ratio of PGⅠand PGⅡ(PGR) was calculated. The morphological changes of gastric mucosa were observed by macroscopy and light microscope. Results ①After modeling, the body weight of rats in the model establishment group was lower than the blank group(P<0.01). Compared with the model group, the body weight of rats in the vitacoenzyme group, the heat-sensitive moxibustion group and the non-heat-sensitive moxibustion group was increased after intervention(P<0.05), and there were no significant differences among the intervention groups(P>0.05). ②Under macroscopy and light microscope, gastric tissue of rats after modeling showed dark red and pale gastric mucosa, lower plica and mucosal congestion. The glands of lamina propria were atro

关 键 词：慢性萎缩性胃炎 热敏灸 穴 中脘 生长激素 胃蛋白酶原 

分 类 号：R245.81[医药卫生—针灸推拿学]