Genome-wide Dissection of Co-selected UV-B Responsive Pathways in the UV-B Adaptation of Qingke  

作　　者：Xingquan Zeng Hongjun Yuan Xuekui Dong Meng Peng Xinyu Jing Qijun Xu Tang Tang Yulin Wang Sang Zha Meng Gao Congzhi Li Chujin Shu Zexiu Wei Wangmu Qimei Yuzhen Basang Jiabu Dunzhu Zeqing Li Lijun Bai Jian Shi Zhigang Zheng Sibin Yu Alisdair R.Fernie Jie Luo Tashi Nyima 

机构地区：[1]State Key Laboratory of Hulless Barley and Yak Germplasm Resources and Genetic Improvement,Lhasa 850002,China [2]Research Institute of Agriculture,Tibet Academy of Agricultural and Animal Husbandry Sciences,Lhasa,Tibet 850002,China [3]Tibet Academy of Agricultural and Animal Husbandry Sciences,Lhasa,Tibet 850002,China [4]Wuhan Metware Biotechnology Co.,Ltd.,Wuhan,China [5]National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research(Wuhan),Huazhong Agricultural University,Wuhan 430070,China [6]IB-UGent Center for Plant Systems Biology,Ghent University,Technologiepark-Zwijnaarde,71,9052 Ghent,Belgium-. [7]Agricultural Resources and Environment Research Institute,Tibet Academy of Agricultural and Animal Husbandry Sciences,Lhasa,Tibet 850002,China [8]Wuhan Igenebook Biotechnology Co.,Ltd.,Wuhan,China [9]Chengdu Life Baseline Technology Co.,Ltd,Chengdu,610041,China [10]Max-Planck-lnstitute of Molecular Plant Physiology,Potsdam-Golm 144776,Germany [11]College of Tropical Crops,Hainan University,Haikou,Hainan 572208,China

出　　处：《Molecular Plant》2020年第1期112-127,共16页分子植物（英文版）

基　　金：This research was supported by the following funding sources:the Tibet Autonomous Region Financial Special Fund 2015CZZX001,2014CZZXQ01,2015ZX001,2017CZZX001/2,and XZNKY-2018-C-021,and the National Key Research Project Fund,China 2018YFD100070 and 2018YFD1000703.

摘　　要：Qingke (Tibetan hulless barley) has long been cultivated and exposed to long-term and strong UV-B radiation on the Tibetan Plateau, which renders it an ideal species for elucidating novel UV-B responsive mechanisms in plants. Here we report a comprehensive metabolite profiling and metabolite-based genome-wide association study (mGWAS) using 196 diverse qingke and barley accessions. Our results demonstrated both constitutive and induced accumulation, and common genetic regulation, of metabolites from different branches of the phenylpropanoid pathway that are involved in UV-B protection. A total of 90 significant mGWAS loci for these metabolites were identified in barley-qingke differentiation regions, and a number of high-level metabolite trait alleles were found to be significantly enriched in qingke, suggesting co-selection of various phenylpropanoids. Upon dissecting the entire phenylpropanoid pathway, we identified some key determinants controlling natural variation of phenylpropanoid content, including three novel proteins, a flavone C-pentosyltransferase, a tyramine hydroxycinnamoyl acyltransferase, and a MYB transcription factor. Our study, furthermore, demonstrated co-selection of both constitutive and induced phenylpropanoids for UV-B protection in qingke.

关 键 词：Tibetan hulless BARLEY UV-B radiation constitutive and induced accumulation PHENYLPROPANOID MYB transcription factor 

分 类 号：Q943.2[生物学—植物学]