基于数据挖掘及网络药理学探究李顺民治疗慢性肾脏病3~5期用药经验及作用机理  

A Discussion on Medication Experience of LI Shunmin for Chronic Kidney Disease at Stage 3 to 5 and on Mechanism of Action Based on Data Mining and Network Pharmacology

作　　者：杨栋[1] 祁爱蓉[1] 李雨彦[1] 戈娜[1] 罗登贵 YANG Dong;QI Airong;LI Yuyan;GE Na;LUO Denggui

机构地区：[1]深圳市中医院,广东深圳518033

出　　处：《新中医》2020年第4期176-180,共5页New Chinese Medicine

基　　金：2014年广东省中医药局建设中医药强省科研项目(20142129);2016年深圳市科创委自由探索项目(JCYJ20160428182213078);2018年国家自然科学基金项目(81704012)。

摘　　要：目的:利用数据挖掘技术总结李顺民教授治疗慢性肾脏病(Chronic kidney disease,CKD)的用药经验,并利用网络药理学技术探究其内在机理。方法:收集笔者2012年1月-2015年12月期间在师承李顺民教授工作中门诊记录的CKD3~5期患者病案,建立Access数据库,利用结构化查询语言(SQL)进行数据处理,统计出李顺民教授治疗CKD3~5期的用药情况,再利用网络药理学技术对其进行分析。结果:共收集CKD3~5期病案5 100份(包括初诊及复诊病案),李顺民治疗CKD3~5期常用的中药有甘草、黄芪、山药、肉苁蓉、大黄、紫苏叶、芡实、生地黄、丹参、冬瓜皮等。其中常用中药组合有黄芪与甘草、黄芪与山药、黄芪与肉苁蓉。通过检索得到黄芪与甘草共130个基因靶标,CKD疾病靶标1 084个,它们之间相同的基因靶标22个;黄芪与山药共365个基因靶标,与CKD疾病靶标相同的有52个;黄芪与肉苁蓉共121个基因靶标,与CKD疾病靶标相同的有28个;黄芪与大黄共83个基因靶点,与CKD疾病靶标相同的有21个;肉苁蓉与甘草共361个基因靶点,与CKD相同的靶标有43个。富集分析后得到它们之间相同的KEGG信号通路有:Wnt信号通路、Notch信号通路及转化生长因子β(TGF-β)、Toll样受体信号通路等。结论:李顺民治疗CKD的用药机理与对Wnt信号通路、PPAR信号通路、Notch信号通路及TGF-β、Toll样受体信号通路的调节相关,这些通路对抗纤维化、抑制炎症及肾性骨病的治疗靶点的调节均综合反映了从脾论治CKD的分子机制。Objective: To summarize the medication experience of Prof. LI Shunmin in treating chronic kidney disease(CKD) with data mining,and explore its internal mechanism with network pharmacology. Methods:The medical records of Prof. LI’s outpatients with CKD at stage 3 to 5(CKD3~5) were collected by the authors during January 2012 to December 2015 to set up an Access database. The medication experience of Prof. LI for CKD3~5 was summarized after data processing by structured query language(SQL) and was analyzed by network pharmacology. Results:A total of 5 100 medical records of CKD3~5,including first-visit and follow-up ones,were collected. The commonly used Chinese herbs by Prof. LI included Radix et Rhizoma Glycyrrhizae,Radix Astragali,Rhizoma Dioscoreae,Herba Cistanches,Radix et Rhizoma Rhei,Folium Perillae,Semen Euryales,Radix Rehmanniae,Radix et Rhizoma Salviae Miltiorrhizae,and Exocarpium Benincasae,and the commonly used combinations were Radix Astragali plus Radix et Rhizoma Glycyrrhizae, Radix Astragali plus Rhizoma Dioscoreae,Radix Astragali plus Herba Cistanches. Through retrieval,there were 1 084 gene targets of CKD,and 130 of Radix Astragali plus Radix et Rhizoma Glycyrrhizae, with 22 identical gene targets between them;there were 365 gene targets of Radix Astragali plus Rhizoma Dioscoreae, with 52 identical with CKD;there were 121 gene targets of Radix Astragali plus Herba Cistanches,with 28 identical with CKD;there were 83 gene targets of Radix Astragali plus Radix et Rhizoma Rhei, with 21 identical with CKD;there were 361 gene targets of Herba Cistanches plus Radix et Rhizoma Glycyrrhizae,with 43 identical with CKD. After enrichment analysis,the same KEGG signaling pathways between them were found to be Wnt,Notch and TGF-β and Toll-like receptor signaling pathways,ect. Conclusion:The mechanism of Prof. LI’s for CKD is related to the regulation of Wnt,Notch and TGF-β and Toll-like receptor signaling pathways,whose regulation against fibrosis,inhibition of inflammation and therapeutic targets of rena

关 键 词：慢性肾脏病(CKD) 数据挖掘 网络药理学 李顺民 用药经验 作用机理 

分 类 号：R692[医药卫生—泌尿科学]