蒲公英黄酮类成分超声辅助半仿生提取及含量测定  

Ultrasonic-assisted semi-bionic extraction and content determination of flavonoid constituent from Taraxacum mongolicum Hand.-Mazz.

作　　者：李艳芝[1] 刘树玲[1] 刘文杰 李爱花 LI Yanzhi;LIU Shuling;LIU Wenjie;LI Aihua(College of Pharmacy,Jining Medical University,Rizhao 276826,China)

机构地区：[1]济宁医学院药学院,日照276826

出　　处：《济宁医学院学报》2020年第1期5-9,共5页Journal of Jining Medical University

基　　金：济宁医学院青年教师科研扶持基金重点项目(JY2016KJ010Z)。

摘　　要：目的 研究蒲公英黄酮类成分超声辅助半仿生提取工艺,并对不同采收时间蒲公英地上部分及根中所含黄酮类成分槲皮素、木犀草素和芦丁进行含量测定。方法 在单因素试验基础上,通过正交试验对提取工艺参数乙醇浓度、料液比、超声时间、超声温度进行优化。采用高效液相色谱法对蒲公英中槲皮素、木犀草素和芦丁进行含量测定。结果 4种因素对蒲公英总黄酮提取率影响大小顺序为乙醇浓度>料液比>超声时间>超声温度,其中乙醇浓度和料液比为显著性影响因素;最佳提取工艺条件为乙醇浓度为95%,料液比为1∶20,超声时间30min,超声温度为40℃。建立了同时测定蒲公英中槲皮素、木犀草素和芦丁的HPLC方法。结论 不同时期蒲公英地上部分及根中槲皮素、木犀草素和芦丁的含量有明显差别,4月份采收的样品含量高于其它采收时间。Objective To optimize the Ultrasonic-assisted semi-bionic extraction process of the flavonoid constituent from Taraxacum mongolicum Hand.-Mazz.and determine the contents of quercetin,luteolin and rutin from aerial part and root of T.mongolicum Hand.-Mazz.in different periods.Methods We optimized the extraction parameter by orthogonal test on the basis of single factor test.The contents of flavonoid was determined by HPLC.Results Among the four factors,the ratio of liquid to solid and ethyl alcohol concentration were significant influencing factors and the order of the more influence factor of the yield was:ethyl alcohol concentration>ratio of liquid to solid>ultrasonic time>ultrasonic temperature.The best extraction condition was as followed:ethyl alcohol concentration(95%)ratio of liquid to solid(1:20),ultrasonic time(30 min),ultrasonic temperature(40℃).The contents of quercetin,luteolin and rutin from the aerial part and root of T.mongolicum Hand.-Mazz.were obviously different in different periods.Conclusion The contents of quercetin,luteolin and rutin from the aerial part and root of T.mongolicum were obviously different in different periods and the content in April is higher than others.

关 键 词：蒲公英 黄酮 半仿生提取 含量测定 

分 类 号：R284.1[医药卫生—中药学]