鸭甲型肝炎病毒的遗传演化分析  

Genetic evolution analysis of duck hepatitis a viruses

作　　者：马秀丽[1] 冯永胜[2] 郝小静[2] 周围 衣服德[2] 代永联[2] 宋玲玲[1] 谢元超 宋敏训[1] 艾武[1] MA Xiuli;FENG Yongsheng;HAO Xiaojing;ZHOU Wei;YI Fude;DAI Yonglian;SONG Lingling;XIE Yuanchao;SONG Minxun;Ai Wu(Shandong Institute of Poultry Science,Shandong Academy of Agricultural Science,The Key lab of poultry disease diagnosis and immunology of Shandong Province,Poultry Breeding Engineering Technology Center of Shandong Province,Jinan 250023,China;Qingdao Institute of animal husbandry and veterinary medicine,Qingdao 266000,China;Shanghai Institute of Materia Medica,Shanghai 201203,China)

机构地区：[1]山东省农业科学院家禽研究所,山东省禽病诊断与免疫重点实验室,山东省家禽育种工程技术研究中心,山东济南250023 [2]青岛市畜牧兽医研究所,山东青岛266000 [3]中国科学院上海药物研究所,上海201203

出　　处：《家禽科学》2020年第2期10-16,共7页Poultry Science

基　　金：山东省重点研发计划项目(2019GNC106044);山东省现代农业产业技术体系项目(SDAIT-11-01);山东省农业科学院农业科技创新工程(CXGC2016A10);山东省农业科学院农业科技创新工程学科团队专项经费(编号CXGC2018E11)

摘　　要：本研究测定了实验室2018~2019年分离的11株鸭甲型肝炎病毒1型(DHAV-1)和37株鸭甲型肝炎病毒3型(DHAV-3)的VP1基因序列,并选取代表毒株与已知阳性血清进行中和试验,分别分析DHAV-1或DHAV-3的遗传和抗原稳定性。结果表明,DHAV-1进化为两个遗传分支,2018~2019年的分离毒株分布在1b分支上,而经典DHAV-1毒株处于不同的1a分支上。DHAV-3进化为两个不同的分支,2018~2019年分离毒株大部分集中在3a分支上,个别毒株分布在3b分支上。DHAV-1或DHAV-3毒株之间均无明显的地理分布差异。血清交叉中和试验结果表明,DHAV-1或DHAV-3同一血清型之间仍具有较好的交叉保护,表明同一血清型的DHAV尚未发生抗原变异。The VP1 coding sequences of 11 DHAV-1 and 37 DHAV-3 isolates(spanning 2018-2019)associated with disease outbreaks were determined.Antigenic stability of representative DHAV-1 and DHAV-3 strains were study by Serum neutralization assay.We demonstrated that DHAV-1 viruses evolved into two genetic lineages,isolated strains spanning 2018-2019 were distributed into the same 1b lineages.DHAV-3 viruses exhibited two distinct lineages,majority isolated strains spanning 2018-2019 were distributed into the same 3a lineages.No Distribution differences were observed for DHAV-1 or DHAV-3 viruses.Antigenic study of representative DHAV-1 and DHAV-3 strains revealed no detectable changes in viral neutralization properties within the same serotype.Results of our experiments described here shall give novel insights into genetic properties and antigenic stability of duck hepatitis A viruses.

关 键 词：鸭甲型肝炎病毒 遗传演化 

分 类 号：S858.325.3[农业科学—临床兽医学]