生长素参与淡黄花百合珠芽发育调节  

Auxin Involved in Bulbil Development of Lilium sulphureum

作　　者：夏丽莎 高华霞 罗睿[1,2] 罗须 杜声珊 Xia Lisha;Gao Huaxia;Luo Rui;Luo Xu;Du Shengshan(College of Life Science,Guizhou University,Guiyang,550025;Key Laboratory of Plant Resources Conservation and Germplasm Innovation in Mountainous Region,Ministry of Education.Guizhou university,Guiyang Guizhou,China,550025)

机构地区：[1]贵州大学生命科学学院,贵阳550025 [2]贵州大学,山地植物资源保护与种质创新省部共建教育部重点实验室,贵阳550025

出　　处：《分子植物育种》2020年第5期1409-1418,共10页Molecular Plant Breeding

基　　金：国家自然科学基金(No.31560081);贵州大学研究生创新基金(研理工2017027)共同资助。

摘　　要：百合是一类重要的经济/观赏植物,包含百合属的多个物种或杂交品种。珠芽繁殖是多种百合的重要营养繁殖途径,但其发育的分子机制少有研究。本研究利用转录组测序技术对来自淡黄花百合(Lilium sulphureum) 5个部位的混合样本进行测序获得6.64 Gb数据,组装得到51 450个Unigene,32 784个Unigene在NR等功能数据库中得到注释(其中487个与生长素代谢及信号转导相关基因同源)。利用表达谱测序技术分别对5个组织样品进行测序共得到46 897个Unigene,其中337个与生长素代谢及信号转导相关基因同源的Unigene在珠芽源器官、发育早期或后期珠芽之间差异表达。结果表明淡黄花百合珠芽发育早期依赖于活跃运输、低失活及逐渐增强的自主合成提高内部生长素含量,后期伴随珠芽内生长点分化形成其自主合成和失活代谢变得更加活跃;生长素浓度的变化引起大量信号转导相关基因表达量发生变化从而调节珠芽的发育。本研究结果为进一步探究生长素在珠芽发育过程中的调节作用提供了依据。Lily is one kind of important economic/ornamental plants that contain multiple species or hybrids of the genus Lilium. The bulbil formation is an important reproductive strategy for some lily. However, the molecular mechanism of its development is seldom studied. In this study, sample mixture of five tissues(Lilium sulphureum)was used to transcriptomic sequencing, and 6.64 Gb data was obtained. The whole of sequences were assembled as51 450 Unigenes, and 32 784 Unigenes were annotated in database(Of these, 487 were homologous to auxin metabolism and signal transduction). Profiling data from five tissues showed that 46 897 Unigenes were expressed in all of the five samples. Totally, 337 Unigenes that related to auxin metalolism and signal transduction were expressed differentially between source organ of bulbil, ealy bulbil, and late bulbil. Results showed that auxin content increasing in early bulbil depended on active transportation, low actively inactivation, and enhanced biosynthesis itself gradually. In late bulbil, the enhancement of auxin biosynthesis endogenously coincided with the formation of SAM(shoot apical meristem) in bulbil. Furthermore, large number of Unigenes expressed differentially during bulbil development that agreed with variation of auxin content. Our results should facilitate researching works to reveal molecular mechanism of Auxin in bulbil development.

关 键 词：淡黄花百合(Liliun sulphureum) 珠芽发育 生长素 差异表达 失活代谢 

分 类 号：S682.29[农业科学—观赏园艺]