植物信号转导对‘夏黑’葡萄冬芽休眠解除的调控  

Regulation of Plant Signal Transduction on Bud Dormancy Release in'Summer Black'Grapevine

作　　者：杨丽丽[1,2] 陈展[2] 赵艳卓[2] 牛早柱 牛帅科[2] 师校欣[1] 杜国强[1] Yang Lili;Chen Zhan;Zhao Yanzhuo;Niu Zaozhu;Niu Shuaike;Shi Xiaoxin;Du Guoqiang(College of Horticulture,Hebei Agricultural University,Baoding,071000;Shijiazhuang Fruit Research Institute,Hebei Academy of Agricultural and Forestry Sciences,Shijiazhuang,050061)

机构地区：[1]河北农业大学园艺学院,保定071000 [2]河北省农林科学院石家庄果树研究所,石家庄050061

出　　处：《分子植物育种》2020年第5期1438-1446,共9页Molecular Plant Breeding

基　　金：国家现代农业产业技术体系项目(CARS-29-3);河北省创新工程项目(F18R06001-5);河北省现代农业产业技术体系(HBCT2018100204)共同资助。

摘　　要：本研究2016年-2017年以生态休眠状态下的‘夏黑’葡萄冬芽为试材,经单芽扦插处理、用转录组方法辅以生理指标测定,探讨了影响葡萄冬芽休眠解除的关键因子。单芽扦插7 d (EB1)和扦插14 d (EB2)后冬芽转录组测序均得到45 Mb以上的Clean Reads,经过滤、拼接、数据库比对均得到25 000以上转录本。从基因表达分析看EB2与EB1相比,1 051个基因上调表达,587个基因下调表达。差异表达基因(DEGs) GO富集分析发现葡萄冬芽自然休眠解除过程中DEGs主要富集在催化活性、结合、代谢过程和单有机体过程,进一步KEGG代谢途径分析发现植物信号转导途径是休眠解除的重要参与环节,尤其赤霉素(gibberellin,GA)和脱落酸(abscisic acid, ABA)代谢与休眠解除关系密切,芽子休眠解除过程中的内源激素变化和信号转导路径上相关基因qRT-PCR也验证了这一点。因此本研究认为内源激素和植物信号转导是调控葡萄冬芽自然休眠解除的重要因子之一。Transcriptome analysis is an useful method to study the mechanism of grape bud dormancy release. In this study, the dormant buds of ecodormancy were measured by transcriptome analysis as the materials to understand the mechanism of bud dormancy release in 2016 a and 2017 a. Single bud cuttage 7 d and 14 d were named EB1 and EB2, respectively. For EB1 and EB2, more than 45 Mb Clean Reads were obtained through transcriptome sequencing, and more than 25 000 transcripts were obtained through filtration, splicing and comparison. Though the analysis of gene expression level, 1 051 genes of EB2 were up-regulated and 587 genes were down-regulated in EB1 vs EB2 comparison. Differentially expressed genes(DEGs) GO enrichment analysis found that DEGs mainly enriched in the catalytic activity, binding, metabolic process and single-organism process in bud dormancy release.Furthermore KEGG pathways analysis found that plant signal transduction played an important role in dormancy release, especially ABA pathway and GA pathway. Subsequently the endogenous hormone level of ABA, GA, IAA and ZR during the dormancy release and genes qRT-PCR related plant signal transduction also proved this point.Therefore, this study speculate that phytohormone levels and plant signal transduction are one of the important factors to regulate the natural dormancy release of grape winter buds.

关 键 词：葡萄 休眠 转录组 植物激素 QRT-PCR 

分 类 号：S663.1[农业科学—果树学]