栎叶绣球‘雪花’再生体系的建立  

Establishment of the Regeneration System of Hydrangea quercifolia'Snow Flake'

作　　者：聂绍虎 刘江淼 张鑫静 王月莹 高永慧 赵惠恩 Nie Shaohu;Liu Jiangmiao;Zhang Xinjing;Wang Yueying;Gao Yonghui;Zhao Huien(Beijing Laboratory of Urban and Rural Ecological Environment,National Engineering Research Center for Floriculture,Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education,Beijing Key Laboratory of Ornamental Plants Germplasm Innovation&Molecular Breeding,College of Landscape Architecture,Beijing Forestry University,Beijing,100083;Xingcheng Agriculture Broadcast and Television School,Xingcheng,125100)

机构地区：[1]北京林业大学园林学院,花卉种质创新与分子育种北京市重点实验室,林木花卉遗传育种教育部重点实验室,国家花卉工程技术研究中心,城乡生态环境北京实验室,北京100083 [2]辽宁省兴城市农业广播电视学校,兴城125100

出　　处：《分子植物育种》2020年第5期1593-1599,共7页Molecular Plant Breeding

基　　金：国家林业草原局引进国际先进林业科学技术项目(2015-4-15)资助。

摘　　要：为获得大量栎叶绣球的无菌苗,本研究以栎叶绣球‘雪花’(Hydrangea quercifolia’Snow Flake’)的叶片为试验材料,通过不同浓度的激素组合处理。结果表明,最适宜的愈伤组织诱导培养基为:MS+1.5 mg/L6-BA+0.2 mg/L NAA,出愈率约为80.7%;最适宜的不定芽分化培养基为:MS+2 mg/L 6-BA+0.1 mg/L IBA,诱导率约为90.3%;最佳的增殖培养基为:MS+2.5 mg/L 6-BA+0.1 mg/L IBA,增殖率约为5.2;最佳的壮苗培养基为:MS+0.5 mg/L 6-BA+0.15/0.2 mg/L IBA,幼苗平均高度约为4.57 cm;最佳的生根培养基为:1/2 MS+0.4 mg/L IBA,平均生根率约为88.3%,平均根长约为6.5 cm。本研究初步建立了栎叶绣球‘雪花’的再生体系,为其扩大繁殖和之后的遗传转化研究提供了帮助。To obtain a large number of aspetic seedlings of Hydrangea quercifolia, we used the leaves of Hydrangea quercifolia ’Snow Flake’ as test materials, treated by different concentrations of hormones. The results showed that the optimum callus induction medium was: MS+1.5 mg/L 6-BA+0.2 mg/L NAA, and the induction rate was about 80.7%. The optimum adventitious shoot differentiation medium was: MS+2 mg/L 6-BA+0.1 mg/L IBA, the induction rate was about 90.3%. The best proliferation medium was: MS+2.5 mg/L 6-BA+ 0.1 mg/L IBA,the proliferation rate was 5.2. The best medium for seedling cultivation was: MS+0.5 mg/L 6-BA+0.15/0.2 mg/L IBA, and the average height of the plant was about 4.57 cm. The best rooting medium was: 1/2 MS+0.4 mg/L IBA,average rooting rate was about 88.3% and the average root length was about 6.5 cm. This study initially established the regeneration system of the Hydrangea quercifolia ’Snow Flake’, which is helpful to the study of expanded reproduction and subsequent genetic transformation.

关 键 词：栎叶绣球(Hydrangea quercifolia) 愈伤组织 植株再生 

分 类 号：S685.99[农业科学—观赏园艺]