氟西汀和帕罗西汀对斑马鱼脑组织5-HT能神经传导系统的影响  

Effects of SSRIs Antidepressants Fluoxetine and Paroxetine on 5-HTergic Neurotransmission System in Zebrafish Brain Tissue

作　　者：陈瑜 张博 李铁军 CHEN Yu;ZHANG Bo;LI Tie-jun(Marine and Fishery Institute of Zhejiang Ocean University,Marine Fisheries Research Institute of Zhejiang Province,Zhoushan 316021,China)

机构地区：[1]浙江海洋大学海洋与渔业研究所浙江省海洋水产研究所

出　　处：《浙江海洋大学学报（自然科学版）》2019年第4期295-302,共8页Journal of Zhejiang Ocean University：Natural Science

基　　金：浙江省自然科学基金(LY19C030001)

摘　　要：为了解SSRI类抗抑郁药物对斑马鱼脑组织5-HT神经传导系统的影响,选取SSRI类抗抑郁药代表药物氟西汀和帕罗西汀,探讨其对斑马鱼脑组织5-HT系统关键功能分子蛋白5-HT1A受体和5-HT转运体(serotonin transporter,SERT)水平的影响。成年斑马鱼氟西汀、帕罗西汀连续水体暴露7 d和14 d,分别于染毒后通过qPCR检测5-HT1A和SERT转录水平,通过western blot检测SERT蛋白表达水平。结果显示,氟西汀和帕罗西汀均可引起5-HT1A、SERT基因转录水平升高,伴有SERT蛋白表达水平降低。本研究结果提示,SSRI类抗抑郁药物氟西汀和帕罗西汀通过引起斑马鱼脑组织中5-HT1A受体和SERT转录水平和表达水平异常,从而干扰脑组织5-HT能神经传导系统的正常功能,进而影响斑马鱼脑组织5-HT能系统的正常生理功能。In order to understand the effect of SSRI antidepressants on the 5-HT neurotransmission system in zebrafish brain tissue,SSRI antidepressants were selected to represent the drugs fluoxetine and paroxetine to explore the key to 5-HT system in zebrafish brain tissue.Effects of functional molecule protein 5-HT1A receptor and 5-HT transporter(SERT)levels.Adult zebrafish fluoxetine and paroxetine were exposed to continuous water for 7 days and 14 days.The levels of 5-HT1A and SERT were detected by qPCR after exposure,and the expression level of SERT protein was detected by western blot.The results showed that both fluoxetine and paroxetine caused an increase in the transcription levels of 5-HT1A and SERT genes,accompanied by a decrease in the expression level of SERT protein.The results of this study suggest that the SSRI antidepressants fluoxetine and paroxetine interfere with the normal expression of 5-HT1A receptor and SERT in the brain tissue of zebrafish,thereby interfering with the normality of the 5-HT neurotransmission system in brain tissue.The function,in turn,affects the normal physiological function of the 5-HT system of zebrafish brain tissue,resulting in damage to the health of zebrafish.

关 键 词：斑马鱼 氟西汀 帕罗西汀 5-HT1A SERT 

分 类 号：S949[农业科学—水产养殖]