细粒棘球蚴感染对小鼠肝纤维化及炎症因子水平的影响  

Effects of Echinococcus granulosus infection on hepatic fibrosis and inflammatory factors levels in mice

作　　者：张智伟[1] 张生彬[1] 云哲琳[1] Zhang Zhiwei;Zhang Shengbin;Yun Zhelin(Department of Hepatobiliary Surgery,Baogang Hospital,Inner Mongolia,Baotou 014010,China)

机构地区：[1]内蒙古包钢医院肝胆外科,包头014010

出　　处：《广西医科大学学报》2020年第1期43-47,共5页Journal of Guangxi Medical University

基　　金：国家国际科技合作专项课题资助项目(No.2011DFA32740)。

摘　　要：目的:研究细粒棘球蚴感染对小鼠肝纤维化及炎症因子水平的影响。方法:将20只小鼠随机分为对照组和感染组(每组10只),感染组小鼠腹腔注射200 m L细粒棘球蚴原头节液,感染16周,对照组不作处理。比较两组血清中肝功能指标谷丙转氨酶(GPT)、谷草转氨酶(GOT)及纤维化指标层黏连蛋白(LN)、透明质酸酶(HA)水平;苏木精-伊红(HE)染色法观察肝组织形态学特征;实时荧光定量PCR(qPCR)法检测肝组织中炎症因子白细胞介素(IL)-17A、IL-6、γ干扰素(IFN-γ)、人单核细胞趋化蛋白-1(MCP-1)、肿瘤坏死因子-α(TNF-α)mRNA相对表达量。结果:感染组血清GOT、GPT、LN、HA水平显著高于对照组(P<0.05)。HE染色结果显示,感染组小鼠肝组织中汇管区有大量纤维组织增生。感染组小鼠肝组织中IL-17A、IL-6、IFN-γ、MCP-1和TNF-αm RNA相对表达量均显著高于对照组(P<0.05或P<0.01)。结论:细粒棘球蚴感染可导致小鼠肝纤维化的发生,感染期间炎症因子表达水平增高。Objective:To study the effects of Echinococcus granulosus infection on liver fibrosis and inflammatory factors levels in mice.Methods:Twenty mice were randomly divided into a control group and an infected group(10 in each group).The mice in the infected group were intraperitonealy injected with 200 mL Echinococcus granulosus cyst fluid for 16 weeks,while those in the control group were not treated.The liver function indexes such as glutamic pyruvic transaminase(GPT)and glutamic oxaloacetic transaminase(GOT),and liver fibrosis indexes such as laminin(LN)and hyaluronanase(HA)levels in serum were compared.Hematoxylin-eosin(HE)staining was performed to observe the morphological characteristics of liver tissues.Real-time fluorescence quantitative PCR was used to determine the mRNA expressions of inflammatory factors including interleukin(IL)-17,IL-6,γinterferon(IFN-γ),human monocyte chemoattractant protein-1(MCP-1)and tumor necrosis factor-α(TNF-α).Results:The contents of GOT,GPT,LN and HA in serum as well as the m RNA levels of IL-17 A,IL-6,IFN-γ,MCP-1 and TNF-αin the infected group were significantly higher than those in the control group(P<0.05 or P<0.01).HE staining revealed that there were a lot of fibrous tissue hyperplasia in the manifold area of liver tissues in the infected group.Conclusion:Echinococcus granulosus infection can induce hepatic fibrosis and the inflammatory factors are highly expressed during infection.

关 键 词：细粒棘球蚴感染 小鼠 肝纤维化 炎症因子 

分 类 号：R532.32[医药卫生—内科学]