D型多杀性巴氏杆菌和绵羊肺炎支原体引起山羊的混合感染  

Mixed Infections of Serotype D Pasteurella multocida and Mycoplasma ovipneumoniae in Goats

作　　者：吴翠兰 刘琰 李军[1,2] 陶立 李常挺[1,2] 彭昊 潘艳 WU Cui-lan;LIU Yan;LI Jun;TAO Li;LI Chang-ting;PENG Hao;PAN Yan(Guangxi Veterinary Research Institute,Nanning,Guangxi,530001,China;Guangxi Key Laboratory of Veterinary Biotechnology,Nanning,Guangxi,530001,China;Animal Husbandry and Aquatic Veterinary Station,Liusanjie Toum,Hechi,Guangxi,China)

机构地区：[1]广西兽医研究所,广西南宁530001 [2]广西兽医生物技术重点实验室,广西南宁530001 [3]河池市宜州区刘三姐镇畜牧水产兽医站,广西河池546300

出　　处：《动物医学进展》2020年第3期57-61,共5页Progress In Veterinary Medicine

基　　金：广西科技重大专项(桂科AA17204057);广西重点研发计划(桂科AB16380106,AB18221074);国家现代农业产业体系广西肉牛肉羊产业创新团队建设(nycytxgxcxtd-09-05);崇左市科技重大专项(崇科FC2018008)。

摘　　要：2018年9月广西某羊场部分山羊发生流涕、咳嗽、呼吸困难和体温升高等临床症状的疾病,为确诊发病原因并提供治疗方案,采用病原分离培养、PCR扩增鉴定的方法进行诊断,并对分离菌进行生化鉴定、致病性试验和药敏试验。结果显示,病料在血平板有圆形的小菌落生长,革兰阴性小球短杆菌,而在PPLO培养基上不生长;病料的PCR扩增结果显示绵羊肺炎支原体和多杀性巴氏杆菌均为阳性;所分离到的病原菌经生化鉴定,该菌符合多杀性巴氏杆菌的特性;用多杀性巴氏杆菌种属和D型多杀性巴氏杆菌特异性引物扩增为阳性;致病性试验显示该菌对小鼠有很强的致病性;药敏试验显示该菌对头孢他啶、头孢噻肟、氧氟沙星高度敏感,对复方新诺明、强力霉素、红霉素、青霉素为耐药。结果表明该病是由绵羊肺炎支原体和D型多杀性巴氏杆菌混合感染引起。In September 2018,some goats became ill with runny nose,cough,dyspnea and elevated body temperature in a goat farm of Guangxi.In order to diagnose the cause of disease and provide treatment scheme,the samples were collected and cultured,identified by PCR amplification.Biochemical characteristics,pathogenicity test and drug sensitivity analysis were perfomed on the isolated pathogenic bateria.The results showed that the cultured bacteria formed typical round drip colonies on blood agar.The Gram staining revealed Gram negative short rod bacteria.But nothing grew on PPLO mmidium.The PCR results showed that both Mycoplasma ovipneumoniae and Pasteurella multocida were positive.The biochemical tests of isolated strain showed that it accorded with characteristics of Pasteurella multocida.The PCR with spectific primers of Pasteurella multocida and serotype D Pasteurella multocida showed positive results.The pathogenicity test showed that this strain could kill mice.The drug susceptibility test showed that the bacterium was highly sensitive to ceftazidime,cefotaxime and ofloxacin.And it was resistant to trimethoprim,doxycycline,erythromycin and penicillin.The results indicated that the disease is caused by mixed infections of serotype D Pasteurella multocida and Mycoplasma ovipneumoniae.

关 键 词：绵羊肺炎支原体 D型多杀性巴氏杆菌 混合感染 致病性 药敏试验 

分 类 号：S852.612[农业科学—基础兽医学]