微生物絮凝剂产生菌的选育及发酵罐放大试验研究  

Screening and Scale-up Fermention Test of Microbial Flocculant-Producing Strain

作　　者：司晓光 张晓青 杜瑾 张爱君 曹军瑞 SI Xiaoguang;ZHANG Xiaoqing;DU Jin;ZHANG Aijun;CAO Junrui(The Institute of Seawater Desalination and Multipurpose Utilization,MNR,Tianjin 300192,China)

机构地区：[1]自然资源部天津海水淡化与综合利用研究所,天津300192

出　　处：《化学与生物工程》2020年第1期34-37,共4页Chemistry & Bioengineering

基　　金：中央公益性科研院所基本科研业务费专项资金项目(K-JBYWF-2019-QR02);系统生物工程教育部重点实验室开放基金项目(20190601)。

摘　　要：以产微生物絮凝剂的芽孢杆菌DHS-63为出发菌株,采用常压室温等离子体(ARTP)诱变系统进行诱变,经筛选及稳定性分析得到一株遗传稳定的突变株A265,摇瓶发酵絮凝剂产量达到1.08 g·L-1,比出发菌株提高12.9%。对该菌株进行发酵罐放大试验,10 L发酵罐絮凝剂产量为1.64 g·L-1,发酵时间48 h,比出发菌株缩短了10 h;70 L发酵罐絮凝剂产量为1.55 g·L-1,发酵时间44 h,比10 L发酵罐发酵时间缩短了4 h。Using a microbial flocculant-producing strain Bacillus sp.DHS-63 as the starting strain,we obtained a mutant strain A265 with high genetic stability by atmospheric room temperature plasma(ARTP)mutagenesis.Cultured in shake flask,the flocculant yield of strain A265 is 1.08 g·L-1,which is 12.9%higher than that of the starting strain.In 10 L fermentor scale-up tests,the flocculant yield is 1.64 g·L-1,and the fermentation period is 48 h,which is 10 h shorter than that of the starting strain.In 70 L fermentor pilot scale tests,the flocculant yield is 1.55 g·L-1,and the fermentation period is 44 h,which is 4 h shorter than that of 10 L fermentor.

关 键 词：絮凝剂 育种 发酵罐 放大试验 

分 类 号：X703.1[环境科学与工程—环境工程]