江苏省某猪场猪链球菌分离鉴定及致病特性分析  

Isolation and identification of different capsular types of Streptococcus suis on a swine farm in Jiangsu Province and the pathogenicity characteristics of the bacteria

作　　者：周俊明[1] 王丹丹[1] 倪艳秀[1] 祝昊丹[1] 孙珂 李彬[1] 俞正玉[1] 吕立新[1] 何孔旺[1] ZHOU Junming;WANG Dandan;NI Yanxiu;ZHU Haodan;SUN Ke;LI Bin;YU Zhengyu;LYU Lixin;HE Kongwang(Institute of Veterinary Medicine,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China;College of Animal Science and Technology,Jilin Agricultural University,Changchun 130118,China)

机构地区：[1]江苏省农业科学院兽医研究所,江苏南京210014 [2]吉林农业大学动物科学技术学院,吉林长春130118

出　　处：《畜牧与兽医》2020年第1期85-89,共5页Animal Husbandry & Veterinary Medicine

基　　金：国家重点研发计划(2018YFD0500101);江苏省生猪产业技术体系生物安全创新团队(SXGC(2017)263);公益性行业(农业)科研专项经费(201303041)。

摘　　要：为了探明2018年3月江苏省某规模化猪场发病猪的病原,本研究对发病猪进行采样和细菌分离,并对细菌进行了PCR鉴定、多位点序列分型(MLST)及小鼠致病力分析。结果发现:在该场4份发病猪组织中分离到猪链球菌2型(SS2)、猪链球菌9型(SS9)菌株各2株,MLST分析显示分离的SS2菌株属于ST7,SS9菌株属于ST243,SS2、SS9分离菌株的毒力基因表现型分别为mrp^+epf^+sly^+orf2^+sao^+fbps^+gdh^+和mrp^-epf^-sly^-orf2^+sao^-fbps^+gdh^+。动物试验显示其中3株猪链球菌分离菌株对BALB/c小鼠有较强致病力。研究结果为该养殖场猪链球菌感染防控措施的制定提供了重要参考。To find out the pathogens of diseased pigs on a large-scale farm in Jiangsu Province in March 2018, four strains belonging to two serotypes of Streptococcus suis were isolated from the tissues of the pigs by continuous sampling.PCR identification, multilocus sequence typing(MLST) analysis, and test of pathogenicity to mice were performed on the strains.The results showed that two isolated strains of Streptococcus suis serotype 2(SS2) belonged to ST7, and another two isolated strains of Streptococcus suis serotype 9(SS9) belonged to ST243.The virulence genotyping analysis showed that the two SS2’s profiles were mrp^+epf^+sly^+or f2^+sao^+fbps^+gdh^+, and the two SS9’s were mrp^-epf^-sly^-or f2^+sao^-fbps^+gdh^+.The animal infection experiment showed that three of the four above-mentioned isolated strains were pathogenic to mice.The present study provided a useful reference for timely prevention and control of infection with and spread of Streptococcus suis on the pig farm.

关 键 词：猪链球菌 血清型 ST型 毒力 

分 类 号：S852.611[农业科学—基础兽医学]