抗黄萎病番茄种质材料的筛选  

Screening of Tomato Germplasm against Verticillium Wilt

作　　者：王明耀[1] 王学颖[1] 鹿秀云[2] 张桂海 张钊[1] 黄志辉 田庆武[1] 王长浩[1] 马德明[1] WANG Ming-yao;WANG Xue-ying;LU Xiu-yun(Vegetable Research Institute of Langfang Academy of Agricultural and Forestry Sciences,Langfang,Hebei 065000;Plant Protection Institute,Hebei Academy of Agriculture and Forestry Sciences,Baoding,Hebei 071000)

机构地区：[1]廊坊市农林科学院蔬菜研究所,河北廊坊065000 [2]河北省农林科学院植物保护研究所,河北保定071000

出　　处：《安徽农业科学》2020年第6期48-49,92,共3页Journal of Anhui Agricultural Sciences

基　　金：河北省科技计划项目“番茄属茄子抗病砧木种质资源创新”(1018072-8)。

摘　　要：通过对117份番茄种质材料(品种或品系)断根后分别接种1×10^7番茄黄萎病菌、1×10^7茄子黄萎病菌、清水(对照),并进行田间检测,筛选出高抗黄萎病的番茄种质材料15份、感黄萎病的种质材料2份;经对17份材料29个样本的DNA分子检测,结果与田间抗性表现的吻合度为75.86%。该研究为筛选适合茄子生产用抗黄萎病砧木(番茄)育种提供抗源材料,并为实验室番茄抗性DNA分子检测提供理论依据。117 tomato germplasm materials(varieties or strains)were inoculated with 1×10^7 tomato verticillium,wilt bacteria 1×10^7 eggplant verticillium,wilt bacteria water(control)and field test,respectively,15 tomato germplasm materials with high resistance to verticillium wilt and 2 tomato germplasm materials with high resistance to verticillium wilt were selected.Through the DNA molecular detection of 29 samples of 17 materials,the result and the resistance performance in the field were 75.86%.The study provides anti-source materials for screening rootstock breeding suitable for eggplant production,and theoretical basis for laboratory tomato resistant DNA molecular detection.

关 键 词：番茄种质 抗黄萎病筛选 DNA抗性分子检测 

分 类 号：S641.2[农业科学—蔬菜学]