临床抗菌药对兔源沙门氏菌的体外抑菌效果  

Antimicrobial Effects of Clinical Antibacterial Drugs on Salmonella from Rabbit in vitro

作　　者：明月月 吴群 牛金利 丁月霞[1] MING Yue-yue;WU Qun;NIU Jin-li(Agricultural College,Guangdong Ocean University,Zhanjiang,Guangdong 524088)

机构地区：[1]广东海洋大学农学院,广东湛江524088

出　　处：《安徽农业科学》2020年第6期74-76,共3页Journal of Anhui Agricultural Sciences

基　　金：广东海洋大学创新强校工程项目(Q18290);广东海洋大学科研启动项目(R17020)。

摘　　要：为了解临床常用抗菌药对兔源沙门氏菌的体外抑菌效果,采用微量肉汤二倍稀释法测定临床常用8种抗菌药物对病原菌的最小抑菌浓度(MIC),采用棋盘稀释法进行体外联合药敏试验测定病原菌部分的抑菌浓度指数(FIC)。结果表明,分离鉴定病原菌为沙门氏菌;庆大霉素的抑菌效果最强(0.125μg/mL),其次为氧氟沙星(0.250μg/mL)、恩诺沙星(0.500μg/mL),磺胺嘧啶钠(8.000μg/mL)、磺胺间甲氧嘧啶与阿米卡星(均为16.000μg/mL)抑菌效果较差,而阿莫西林与青霉素的抑菌效果最差(均为128.000μg/mL)。联合药敏试验结果表明,阿莫西林与氧氟沙星、庆大霉素与氧氟沙星联用呈协同作用,阿米卡星与磺胺嘧啶钠、阿米卡星与磺胺间甲氧嘧啶、恩诺沙星与阿米卡星联用呈无关作用,氧氟沙星与磺胺间甲氧嘧啶、青霉素与阿米卡星、庆大霉素与磺胺嘧啶钠联用呈拮抗作用。In order to understand antimicrobial effect of the clinical commonly used antimicrobial agents on salmonella from rabbit in vitro,we used eight kinds of antimicrobial drugs against pathogens to investigate the minimum inhibitory concentration(MIC)of the clinical commonly,and the fractional inhibitory concentration(FIC index)of some antimicrobial agents against pathogens was determined by the chessboard dilution method of microbroth.The results showed that the isolated and identified pathogenic was Salmonella sp..And antibacterial drugs of gentamicin performed well with MIC of 0.125μg/mL;followed by xfloxacin(0.250μg/mL)and enrofloxacin(0.500μg/mL),the sulfadiazine sodium(8.000μg/mL),sulfamonomethoxine and amikacin(both 16.000μg/mL)have even lower antimicrobial effect;amoxicillin and penicillin showed the worst antimicrobial effect,with MIC both of 128.000μg/mL.By combining with drug sensitive test results,the combination of amoxicillin and ofloxacin,gentamicin and ofloxacin showed a synergy effect.But the combination of amikacin and sulfadiazine sodium,amikacin and sulfamonomethoxine,enrofloxacin and amikacin showed no correlation.Penicillin and amikacin,gentamicin and sulfadiazine sodium had a combination role in antagonism.The combination of xfloxacin and sulfamonomethoxine,penicillin and amikacin,gentamicin and sulfadiazine sodium showed antagonism.

关 键 词：兔源沙门氏菌 抗菌药 最小抑菌浓度(MIC) 联合药敏试验 

分 类 号：S855.1[农业科学—临床兽医学]