维生素C对单试剂氧化酶法检测总胆固醇和三酰甘油的干扰分析  

Interference of vitamin C to oxidase assay with single reagent in the determination of total cholesterol and triglyceride

作　　者：李得娟 唐云芳 罗静萍 巩琳莉 李红[1,6] 张瑶 梁珊珊 贺勇 LI Dejuan;TANG Yunfang;LUO Jingping;GONG Linli;LI Hong;ZHANG Yao;LIANG Shanshan;HE Yong(Department of Laboratory Medicine,West China Hospital of Sichuan University,Chengdu,Sichuan 610041,China;Department of Clinical Laboratory,Qinghai Province Cardiovascular and Cerebrovascular Disease Hospital,Xining,Qinghai 810000,China;Department of Clinical Laboratory,Yun Country People′s Hospital,Lincang,Yunnan 675800,China;Department of Clinical Laboratory,the third People′s Hospital of Chengdu Wuhou District,Chengdu,Sichuan 610041,China;Department of Clinical Laboratory,Chengdu Civil Aviation Medical Center,Chengdu,Sichuan 610041,China;Department of Clinical Laboratory,Guangyuan Wangcang Traditional Chinese Medicine Hospital,Guangyuan,Sichuan 628200,China;Department of Clinical Laboratory,Renshou Country Traditional Chinese Medicine Hospital,Meishan,Sichuan 620500,China)

机构地区：[1]四川大学华西医院实验医学科,四川成都610041 [2]青海省心脑血管病专科医院检验科,青海西宁810000 [3]云南省云县人民医院检验科,云南临沧675800 [4]四川省成都市武侯区第三人民医院检验科,四川成都610041 [5]四川省成都市民用航空医学中心检验科,四川成都610041 [6]四川省广元市旺苍中医院检验科,四川广元628200 [7]四川省仁寿县中医医院检验科,四川眉山620500

出　　处：《国际检验医学杂志》2020年第7期787-790,共4页International Journal of Laboratory Medicine

基　　金：四川省科技计划项目(2019YFS0320)。

摘　　要：目的分析不同维生素C(VC)浓度对单试剂氧化酶法检测总胆固醇(CHOL)和三酰甘油(TG)的干扰影响,确定影响临床应用价值的最低VC干扰浓度。方法收集覆盖CHOL、TG检测线性范围的新鲜血清样本各10份,制备不同浓度梯度的VC干扰管,单试剂氧化酶法测定CHOL和TG,计算加入不同梯度浓度的VC前后CHOL、TG的差值及相对偏差,使用国家卫生行业标准(WS/T403-2012)的允许总误差判断偏差是否具有临床意义,确定不同CHOL、TG浓度对VC影响检测结果不可接受的浓度。结果VC对单试剂酶法检测CHOL、TG的干扰为负干扰,干扰值与VC干扰浓度呈正相关关系,随着干扰浓度的增加,干扰值越大。相关关系方程式为TG:Y=-1.0021 X(r 2=0.9956);CHOL:Y=-0.997 X-0.0213(r 2=0.9976)。结论VC对单试剂氧化酶法检测CHOL和TG产生负干扰,产生具有临床意义的干扰与VC干扰浓度及CHOL、TG值密切相关。Objective To analyze the influence of the different concentration of vitamin C on total cholesterol(CHOL)and triglyceride(TG)by oxidase assay with single reagent,and confirm the lowest interference concentration of vitamin C which influences clinic application value.Methods Ten blood samples covering in the detection linear range of CHOL and TG were collected,and different concentrations of vitamin C were prepared as interfering tubes,the concentration of CHOL and TG were detected by oxidase assay with single reagent,and calculated the difference value and relative deviation of CHOL and TG before and after adding different concentrations of vitamin C,allowable total error of National industry standard(WS/T403-2012)was used to judge whether the deviation had clinic significance,to determine the different concentrations of vitamin C which were unacceptable for the test results.Results The vitamin C had a negative interference on the test of CHOL and TG by oxidase assay with single reagent,the testing value of CHOL and TG was positively correlated with vitamin C′s concentration,with the increasing of the interference concentration,the interference value increasing.The correlation equations of TG and CHOL were respectively Y=-1.0021 X(r 2=0.9956)and Y=-0.997 X-0.0213(r 2=0.9976).Conclusion The different concentrations of vitamin C have a negative interference on the value of CHOL and TG by oxidase assay with single reagent,and whether the interference has clinical significance,it depends on the concentrations of vitamin C,CHOL and TG.

关 键 词：总胆固醇 三酰甘油 维生素C 氧化酶法 单试剂 允许总误差 

分 类 号：R446.112[医药卫生—诊断学]