雄激素受体αA基因甲基化和tHcy对颅内动脉狭窄的影响  

Effects of methylation of androgen reciptor-αA gene and total homocysteinet on intracranial arterial stenosis

作　　者：黄毓娟 万里 杨志宏 沈舒文 HUANG Yujuan;WAN Li;YANG Zhihong;SHEN Shuwen(School of Basic Medicine,Shaanxi University of Chinese Medicine,Xianyang 712046,China;Department of Neurology,Xi'an Daxing Hospital,Xi'an 710016,China;Department of Encephalopathy,Affiliated Hospital of Shaanxi University of Chinese Medicine,Xianyang 712046,China;School of Traditional Chinese Medicine,Shaanxi University of Chinese Medicine,Xianyang 712046,China)

机构地区：[1]陕西中医药大学基础医学院,陕西咸阳712046 [2]西安大兴医院神经内科,陕西西安710016 [3]陕西中医药大学附属医院脑病科,陕西咸阳712046 [4]陕西中医药大学中医系,陕西咸阳712046

出　　处：《中国医学物理学杂志》2020年第3期311-316,共6页Chinese Journal of Medical Physics

基　　金：全国名老中医药专家沈舒文教授传承工作室建设项目(国中医药人教发【2016】42号)。

摘　　要：目的:主要探讨人雄激素受体αA(Androgen reciptor-αA,AR-αA)基因启动子区甲基化状态与高分辨率磁共振成像下,以大脑中动脉M1段不同信号斑块为例,探讨颅内动脉狭窄斑块临床与分子生物学的关系。方法:在缺血性脑卒中患者中,行头颅高分辨率磁共振成像检查,入选69例大脑中动脉M1段存在斑块且有神经系统缺损症状的患者和30例对照组,评估颅内动脉斑块信号。采用甲基化特异性聚合酶链反应(MSP)检测静脉血AR-αA基因启动子区甲基化状态及荧光法测定血清总同型半胱氨酸(t Hcy)水平。结果:斑块高信号或混杂信号组42例AR-αA基因启动子区甲基化率为78.6%;等信号或低信号组27例AR-αA基因启动子区甲基化率为70.4%;30例对照者中AR-αA基因启动子甲基化率为26.6%;3组甲基化率差异有显著性意义(P<0.05)。同时,实验组t Hcy水平显著高于对照组(P<0.05),AR-αA基因启动子区甲基化程度与t Hcy相关系数为r=0.549。结论:颅内动脉高信号斑块的不稳定性程度增加,与tHcy成正相关,更易引起颅内动脉狭窄。Objective To discuss the relationship between intracranial arterial stenosis plaques in clinic and molecular biology under the methylation state of androgen reciptor-αA(AR-αA)gene promoter region and high-resolution magnetic resonance imaging by taking plaques of different signals in M1 segment of middle cerebral artery as example.Methods Patients with ischemic stroke were detected by high-resolution magnetic resonance imaging.A total of 69 patients with plaques in M1 segment of middle cerebral artery and neurological defect symptoms were enrolled in the study,and 30 cases in control group.Intracranial arterial plaque signals were evaluated.Methylation specific polymerase chain reaction(MSP)was used for detecting methylation state of AR-αAgene promoter region of venous blood,and fluorescence method for determining the level of serum total homocysteinet(t Hcy).Results The methylation rate of AR-αAgene promoter region in the high signal or mixed signal group(n=42)was 78.6%,and that was 70.4%in iso-signal or low signal group(n=27)and 26.6%in control group(n=30).There were statistical differences in methylation rate among 3 groups(P<0.05).Meanwhile,the level of t Hcy in experimental group was significantly higher than that in control group(P<0.05).The correlation coefficient r between the methylation of AR-αAgene promoter region and t Hcy was 0.549.Conclusion The instability of high signal plaques in intracranial artery is increased and is positively related to tHcy,which is more likely to lead to intracranial artery stenosis.

关 键 词：雄激素受体αA 甲基化 总同型半胱氨酸 颅内动脉狭窄 

分 类 号：R35[医药卫生—基础医学] R445.2