小窝蛋白Cav-1介导人参皂苷Rb1对小鼠脑缺血再灌注损伤的脑保护作用  

Ginsenoside Rb1 protects brain through Cav-1 for mice with cerebral ischemia-reperfusion injury

作　　者：张高娇 王芯芯 周佳 王雷雷[3] 戴勤学[3] ZHANG Gaojiao;WANG Xinxin;ZHOU Jia;WANG Leilei;DAI Qinxue(Department of Anesthesiology,Wenzhou Hospital of Integrated Traditional Chinese and Western Medicine,Wenzhou 325000,Zhejiang,China;Operating Room,Wenzhou Hospital of Integrated Traditional Chinese and Western Medicine,Wenzhou 325000,Zhejiang,China;The First Affiliated Hospital of Wenzhou Medical University,Wenzhou 325000,Zhejiang,China)

机构地区：[1]温州市中西医结合医院麻醉科,浙江温州325000 [2]温州市中西医结合医院手术室,浙江温州325000 [3]温州医科大学附属第一医院麻醉科,浙江温州325000

出　　处：《中国临床药理学与治疗学》2020年第3期265-270,共6页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：国家自然科学基金资助项目(81704180);温州市科技局科技计划项目(Y20170143,Y20140543)。

摘　　要：目的:探讨小窝蛋白Cav-1介导人参皂苷Rb1对小鼠脑缺血再灌注损伤的脑保护作用。方法:将120只C57BL/6小鼠按随机数字表法分成假手术组、模型组、人参皂苷Rb1组、人参皂苷Rb1+Cav-1 siRNA组、人参皂苷Rb1+siNC组,每组24只。采用大脑中动脉闭塞(middle cerebral artery occlusion,MCAO)法建立小鼠脑缺血再灌注损伤模型。人参皂苷Rb1组在造模后即刻给予人参皂苷Rb1(40 mg/kg,i.p.),假手术组和模型组给予等量生理盐水。人参皂苷Rb1+Cav-1 siRNA组和人参皂苷Rb1+siNC组在造模前24 h分别侧脑室注射Cav-1 siRNA和siNC,其他操作同人参皂苷Rb1组。再灌注24 h时测各组小鼠神经行为学评分,然后每组各取6只小鼠分别检测脑组织含水量、脑梗死体积,大脑皮质半暗带区Cav-1 mRNA和Cav-1、Bcl2、Bax蛋白表达量。结果:与假手术组相比,模型组小鼠的神经行为学评分、脑梗死体积、脑组织含水量明显增加(P<0.05),Cav-1 mRNA和Cav-1蛋白表达量、Bcl-2/Bax比值明显减少(P<0.05)。与模型组相比,人参皂苷Rb1组小鼠的神经行为学评分、脑梗死体积、脑组织含水量明显减少,Cav-1 mRNA和Cav-1蛋白表达量、Bcl-2/Bax比值明显增加(P<0.05);与人参皂苷Rb1组相比,人参皂苷Rb1+Cav-1 siRNA组神经行为学评分、脑梗死体积、脑组织含水量明显增加,且Cav-1 mRNA和Cav-1蛋白表达量、Bcl-2/Bax比值明显减少(P<0.05)。结论:人参皂苷Rb1对脑缺血再灌注损伤小鼠具有脑保护作用。而Cav-1 siRNA使小鼠脑组织中Cav-1蛋白表达下降后,可以明显逆转人参皂苷Rb1的脑保护作用,说明Cav-1蛋白介导了人参皂苷Rb1对脑缺血再灌注损伤小鼠的脑保护作用。AIM:To investigate the protective effect of ginsenoside Rb1 on brain through Cav-1 in mice with cerebral ischemia-reperfusion injury.METHODS:One hundred and twenty C57/B6 mice were randomly divided into sham operation group,model group,model+ginsenoside Rb1 group,ginsenoside Rb1+Cav-1 siRNA group,ginsenoside Rb1+siNC group,24 in each group.The model of cerebral ischemia-reperfusion injury in mice was established by middle cerebral artery occlusion(MCAO).The ginsenoside Rb1 group received intraperitoneally injection of ginsenoside Rb1(40 mg/kg);the sham operation group and model group were intraperitoneally injected with an equal amount of physiological saline immediately after modeling.For the ginsenoside Rb1+cav-1 siRNA group and the ginsenoside Rb1+siNC group,cav-1 siRNA and siNC were injected into the lateral ventricle 24 h before molding,respectively,and the other operations were the same as the ginsenoside Rb1 group.The neurobehavioral scores of the mice in each group were measured at 24 h after reperfusion,and the water content of brain tissue,cerebral infarction volume,Cav-1 mRNA and Cav-1,Bcl-2 and Bax protein expressions in the cerebral cortex penumbra were measured in each group.RESULTS:Compared with the sham operation group,the neurobehavioral scores,cerebral infarction volume and brain tissue water content in the model group were significantly increased(P<0.05),and the expressions of Cav-1 mRNA and Cav-1 protein,and the Bcl-2/Bax ratio were significantly decreased(P<0.05).Compared with the model group,the neurobehavioral scores,cerebral infarction volume and brain tissue water content in the ginsenoside Rb1 group were significantly decreased,and the expressions of Cav-1 mRNA and Cav-1 protein,and the Bcl-2/Bax ratio were significantly increased(P<0.05).Compared with the ginsenoside Rb1 group,the neurobehavioral scores,cerebral infarction volume and brain tissue water content in the ginsenoside Rb1+cav-1 siRNA group were significantly increased,and the expressions of Cav-1 mRNA and Cav-1 protein,and th

关 键 词：脑缺血再灌注损伤 人参皂苷RB1 Cav-1 神经保护 

分 类 号：R965.2[医药卫生—药理学]