利用mtCOI PCR-RFLP技术鉴别草地贪夜蛾水稻型和玉米型  

Rapid Differentiation of Corn- and Rice-Strain of Spodoptera furgiperda using mtCOI PCR-RFLP Technique

作　　者：王鹏 孔宝玉 朱军生 国栋 孔子艺 胡磊 褚栋 Wang Peng;Kong Baoyu;Zhu Junsheng;Guo Dong;Kong Ziyi;Hu Lei;Chu Dong(College of Plant Health and Medicine,Qingdao Agricultural University/Key Laboratory of Integrated Crop Pest Management of Shandong Province,Qingdao 266109,China;Shandong Provincial Plant Protection General Station,Jinan 250100,China)

机构地区：[1]青岛农业大学植物医学学院/山东省植物病虫害综合防控重点实验室,山东青岛266109 [2]山东省植物保护总站,山东济南250100

出　　处：《山东农业科学》2020年第3期19-23,共5页Shandong Agricultural Sciences

基　　金：山东省现代农业产业技术体系水稻创新团队项目(SDAIT-17-07);山东省重点研发计划项目(2017GNC11111);山东省一流学科建设项目。

摘　　要：本研究基于mtCOI基因,利用相关软件及系统发育分析,筛选鉴别水稻型和玉米型草地贪夜蛾的酶切位点,并利用PCR-RFLP技术对两者进行鉴定。结果表明:水稻型个体的mtCOI基因序列在154~159 bp处存在SacⅠ酶切位点,玉米型则无该酶切位点;利用mtCOIPCR-RFLP技术可以有效鉴别草地贪夜蛾的水稻型和玉米型。本研究结果可为草地贪夜蛾水稻型、玉米型的快速鉴别及其精准防控提供参考。The restriction enzyme cutting site identifying rice-and corn-strain individuals of Spodoptera frugiperda was screened out by related software and phylogenetic analyses based on mtCOI sequences,and was used to differentiate the two strains.The results showed that the mtCOI sequence of rice-strain individual existed restriction enzyme cutting site of SacⅠat 154~159 bp,but corn-strain individual did not have the above site.The rice-and corn-strain individuals of S.frugiperda could be effectively differentiated by mtCOI PCR-RFLP technology.The results could provide references for the rapid identification and precision control of S.frugiperda.

关 键 词：草地贪夜蛾 玉米型 水稻型 mtCOIPCR-RFLP 快速区分 

分 类 号：S433.4[农业科学—农业昆虫与害虫防治] Q781[农业科学—植物保护]