美洲大蠊提取物对人胚胎皮肤成纤维细胞增殖的影响  

Effects of periplaneta americana extracts on proliferation of human embryonic skin fibroblasts

作　　者：刘荣荣 李丽娟 王楠 张海珠 Rong-rong Liu;Li-juan Li;Nan Wang;Hai-zhu Zhang(School of Public Health,Dali University,Dali,Yunnan 671000,China;Yunnan Provincial Key Laborator of Entomological Biopharmaceutical,Dali University,Dali,Yunnan 671000,China;Department of Pharmacy and Chemistry,Dali University,Dali,Yunnan 671000,China)

机构地区：[1]大理大学公共卫生学院,云南大理671000 [2]大理大学云南省昆虫生物医药研发重点实验室,云南大理671000 [3]大理大学药学与化学学院,云南大理671000

出　　处：《中国现代医学杂志》2020年第5期27-32,共6页China Journal of Modern Medicine

基　　金：2017年省级大学生创新创业训练计划建设项目(No:S-CXCY-2017-7);云南省昆虫生物医药研发重点实验室研究项目(No:2015014)。

摘　　要：目的研究美洲大蠊提取物Ento-A、Ento-B、Ento-C、Ento-D、Ento-E、Ento-F对体外培养人胚胎皮肤成纤维细胞(CCC-ESF-1)增殖的影响。方法在不同时间用不同浓度的美洲大蠊提取物作用CCCESF-1,同时设不加药的阴性对照组(含等量细胞重悬液)和只加培养液的空白组。结果与阴性对照组比较,3和10μg/ml的Ento-A、Ento-B、Ento-C、Ento-D、Ento-E对CCC-ESF-1细胞的增殖抑制作用效果较好,300μg/ml Ento-F对CCC-ESF-1细胞的增殖有抑制作用。3μg/ml Ento-A作用24 h时对CCC-ESF-1细胞的增殖抑制作用效果最好,3、10μg/ml Ento-B、Ento-C、Ento-D、Ento-E作用72和96 h时对CCCESF-1细胞的增殖抑制作用效果较好,Ento-F对CCC-ESF-1细胞的增殖抑制作用不佳,均弱于其他5种。结论美洲大蠊提取物能抑制体外CCC-ESF-1细胞的增殖。Objective To study the effects of periplaneta americana extracts(Ento-A,Ento-B,Ento-C,Ento-D,Ento-E and Ento-F)on the proliferation of human embryonic skin fibroblasts(CCC-ESF-1)in vitro.Methods MTT assay was used to detect the proliferation of CCC-ESF-1 cells in different concentrations and different action time of periplaneta Americana extracts.At the same time,the negative control group(containing equal amount of cell suspension)and the blank control group with only culture medium were set up.Results Compared with the negative control group,periplaneta americana extracts(Ento-A,Ento-B,Ento-C,Ento-D and Ento-E)had better inhibitory effect on the proliferation of CCC-ESF-1 cells at low concentrations of 3 and 10μg/ml.Periplaneta americana extract Ento-F had inhibitory effect on the proliferation of CCC-ESF-1 cells at medium concentrations of 300μg/ml.Ento-A had the best inhibitory effect on the proliferation of CCC-ESF-1 cells at low concentration of 3μg/ml at 24 h.Ento-B,Ento-C,Ento-D and Ento-E had the best inhibitory effect on the proliferation of CCC-ESF-1 cells at low concentration of 3μg/ml and 10μg/ml at 72 h and 96 h.Ento-F had no inhibitory effect on the proliferation of CCCESF-1 cells and was obviously weaker than the other five species.Conclusions Periplaneta Americana extracts can inhibit the proliferation of human embryonic skin fibroblasts(CCC-ESF-1)in vitro.

关 键 词：增生性瘢痕 美洲大蠊提取物 人胚胎皮肤成纤维细胞 细胞增殖 抑制作用 

分 类 号：R619.6[医药卫生—外科学] R285.5[医药卫生—临床医学]