精杞胶囊对环磷酰胺诱导大鼠生精细胞凋亡的抑制作用  

Inhibitory Effect of Jingqi Capsule for Apoptosis of Spermatogenic Cells Induced by Cyclophosphamide in Rats

作　　者：黄佳妮 马红[1] 吕晔[2] 韦敏[1] HUANG Jia-ni;MA Hong;LU Ye;WEI Min(School of Traditional Chinese Medicine and Integrated Traditional Chinese and Western Medicine,Nanjing University of Chinese Medicine,Nanjing(210023;Institute of Botany,Chinese Academy of Sciences,Nanjing(210014))

机构地区：[1]南京中医药大学中医学院·中西医结合学院,南京210023 [2]中国科学院植物研究所,南京210014

出　　处：《中国中西医结合杂志》2020年第3期347-352,共6页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：江苏省2016年第三批省级地方教育项目(No.022062004001);江苏高校优势学科建设工程资助项目(No.苏政办发[2018]87号)。

摘　　要：目的探讨精杞胶囊对环磷酰胺诱导大鼠生精细胞凋亡的抑制作用。方法40只SPF级SD雄性大鼠随机分成5组:正常组、模型组、精杞低剂量组[500 mg/(kg·d)]、精杞高剂量组[1000 mg/(kg·d)]和丙酸睾酮组[5 mg/(kg·d)],每组8只。给药组用药14 d后,除正常组外,其余各组腹腔注射环磷酰胺25 mg/(kg·d)连续5 d,造模期间各给药组延续给药,造模结束后次日处死全部大鼠。HE染色法观察睾丸组织形态;透射电镜观察生精细胞超微结构;ELISA法检测血清睾酮(T)、卵泡生成素(FSH)和黄体生成素(LH)含量及附睾管腔液中唾液酸(SA)的含量;流式细胞仪分析精子细胞凋亡和细胞周期。结果与正常组比较,模型组睾丸曲精细管内各级生精细胞含量较少,结构杂乱,生精细胞核崩解、坏死,线粒体肿胀空泡化,嵴断裂,高尔基复合体结构紊乱。与模型组比较,各给药组病理减轻。与正常组比较,模型组大鼠T、FSH、LH、SA含量及各期细胞存活率、G1期细胞比例降低,凋亡率及S期细胞比例增加(P<0.01)。与模型组比较,给药组T、FSH、LH、SA含量及各期细胞存活率、G2期细胞比例增高,凋亡率及S期细胞比例降低(P<0.05,P<0.01)。与丙酸睾酮组比较,精杞低剂量组的大鼠血清T、FSH、LH及SA含量降低(P<0.01),精杞低、高剂量组细胞存活率下降,早期凋亡率和总凋亡率增加(P<0.05,P<0.01)。结论精杞胶囊对环磷酰胺所诱导的大鼠生精细胞凋亡有抑制作用,其机制可能与调节生殖激素激素水平有关。Objective To investigate the inhibitory effect of Jingqi Capsule(JQC) for spermatogenic cell apoptosis induced by cyclophosphamide in rats. Methods Totally 40 SPF male SD rats were randomly divided into 5 groups: normal group, model group, low-dose JQ group(500 mg·kg-1·d-1), high-dose JQ group(1 000 mg·kg-1·d-1) and testosterone propionate group(5 mg·kg-1·d-1), 8 rats in each group. After 14 days of administration, the groups were intraperitoneally injected with cyclophosphamide 25 mg·kg-1·d-1 for 5 days except the normal group. During the modeling period, all the rats in each group were given the drug continuously, and all the rats were sacrificed the next day after the end of the modeling. The histological morphology of testis was observed by HE staining. The ultrastructure of spermatogenic cells was observed by transmission electron microscopy. The contents of testosterone(T), follicle-stimulating hormone(FSH) and luteinizing hormone(LH) in serum and sialic acid(SA) in epididymal lumen fluid were detected by ELISA. The apoptosis and cell cycle of sperm cells were analyzed by flow cytometry. Results Compared with normal group, the content of spermatogenic cells at all levels in the testicular convoluted tubule in the model group decreased, the structure was disorderly, the spermatogenic nucleus was disintegrated and necrotic, the mitochondria was swelled and vacuolated, the crest was broken, and the golgi complex was disordered. Compared with model group, the pathology of treatment groups was alleviated. Compared with normal group, the contents of T, FSH, LH and SA, the survival rate of cells in each stage and the proportion of cells in G1 phase decreased, while the apoptosis rate and the proportion of cells in S phase increased in model group(P<0.01). Compared with model group, the contents of T, FSH, LH and SA, the survival rate of cells in each stage and the proportion of cells in G2 phase increased, while the apoptosis rate and the proportion of cells in S phase decreased in treatment group(P<0.05,

关 键 词：精杞胶囊 环磷酰胺 生精细胞 细胞凋亡 

分 类 号：R285.5[医药卫生—中药学]