橡胶树种质资源的倍性鉴定  

Ploidy Identification of Germplasm Resources in Hevea brasiliensis(Willd. ex A. Juss.) Müll. Arg.

作　　者：张源源[1,2,3,4,5] 濮寿琴 胡彦师 方家林[1,2,3,4,5] 黄肖 李维国[1,2,3,4,5] 黄华孙 ZHANG Yuanyuan;PU Shouqin;HU Yanshi;FANG Jialin;HUANG Xiao;LI Weiguo;HUANG Huasun(Rubber Research Institute,Chinese Academy of Tropical Agricultural Sciences,Haikou,Hainan 571101,China;State Centre for Rubber Breeding,Haikou,Hainan 571101,China;Rubber Tree Germplasm Repository,Ministry of Agriculture and Rural Affairs,Haikou,Hainan 571101,China;Key Laboratory of Biology and Genetic Resources of Rubber Tree,Ministry of Agriculture and Rural Affairs,Haikou,Hainan 571101,China;State Key Laboratory Incubation Base for Cultivation&Physiology of Tropical Crops,Haikou,Hainan 571101,China;College of Forestry,Hainan University,Haikou,Hainan 570228,China;Engineering Research Center of Rare and Precious Tree Species in Hainan Province,Haikou,Hainan 570228,China)

机构地区：[1]中国热带农业科学院橡胶研究所,海南海口571101 [2]国家橡胶树育种中心,海南海口571101 [3]农业农村部儋州橡胶树种质资源圃,海南海口571101 [4]农业农村部橡胶树生物学与遗传资源利用重点实验室,海南海口571101 [5]省部共建国家重点实验室培育基地-海南省热带作物栽培生理学重点实验室,海南海口571101 [6]海南大学林学院,海南海口570228 [7]海南省热带珍稀名贵树种工程研究中心,海南海口570228

出　　处：《热带作物学报》2020年第3期457-463,共7页Chinese Journal of Tropical Crops

基　　金：中国热带农业科学院基本科研业务费专项资金“橡胶树种质鉴定”(No.1630022017010);现代农业产业技术体系“国家天然橡胶产业技术体系高产品种改良”(No.CARS-34-YZ2)。

摘　　要：倍性鉴定是橡胶树种质资源鉴定评价的基础工作之一。本研究利用流式细胞仪对国家橡胶树种质资源圃内的橡胶树种质资源进行全面的倍性鉴定,对嵌合体的四倍体细胞比例进行分析,并比较不同倍性的气孔性状差异。从5177份野生种质中发现三倍体11份,四倍体1份。从552份魏克汉种质中发现,三倍体9份,四倍体2份,嵌合体14份。14份嵌合体的四倍体细胞比例在49.06%~78.26%之间。不同倍性间的气孔长度、宽度和密度差异均极显著,气孔长度和宽度的排序为四倍体>三倍体>嵌合体,气孔密度的排序相反。研究结果对利用四倍体种质资源开展橡胶树倍性间杂交育种具有重要应用价值。Ploidy of rubber tree germplasm in the Rubber Tree Germplasm Repository and the ratio of tetraploid cells in chimeras were analyzed by flow cytometry, and the stomatal traits of different ploidy were analyzed. Eleven triploids and one tetraploid were found from 5177 1981’IRRDB germplasms, nine triploids and two tetraploid and fourteen chimeras were found from 552 Wickham germplasms. The ratio of tetraploid cells in fourteen chimeras ranged from 49.06% to 78.26%. The difference of stomatal length, stomatal width, and stomatal density between different ploidy was significant(P<0.01), the rank of stomatal length and stomatal width was tetraploid>triploid>chimeras, while the stomatal density was reversed. This study was valuable for the use of tetraploid germplasm resources for cross breeding between the ploidy of rubber trees.

关 键 词：橡胶树 种质资源 多倍体 流式细胞仪 气孔性状 

分 类 号：Q949.748.5[生物学—植物学]