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作 者:赵欣桐 谢新春 张巨明 刘天增 ZHAO Xintong;XIE Xinchun;ZHANG Juming;LIU Tianzeng(College of Forestry and Landscape Architecture,South China Agricultural University,Guangzhou,Guangdong 510642,China)
机构地区:[1]华南农业大学林学与风景园林学院,广东广州510642
出 处:《热带作物学报》2020年第3期489-495,共7页Chinese Journal of Tropical Crops
基 金:广东省科技计划项目(No.2012B020302002);国家自然科学基金项目(No.31502011)。
摘 要:以海滨雀稗(Paspalum vaginatum)的3个品种(Sea Isle 2000、Platinum、Salam)经60Co-γ射线辐射处理的突变体培育的后代为材料,在NaCl胁迫(处理盐浓度设为0、7、21、35 g/L)条件下,通过测定叶片相对含水量、相对电导率、脯氨酸含量、叶绿素含量、根系活力等生理指标的变化来综合评价其耐盐性并筛选出耐盐性较强的品种。结果表明:所有突变材料中PL-40-8对盐胁迫的敏感性小于亲本对照Platinum(PL-CK);叶片相对含水量、叶绿素含量、根系活力均显著高于PL-CK(P<0.05);细胞膜破损的程度小于PL-CK,并且受盐害所产生的脯氨酸上升幅度显著低于PL-CK(P<0.05)。因此,该突变材料可作为耐盐草种进一步选育及推广。The progeny of three species of Paspalum vaginatum(Sea Isle 2000, Platinum, Salam) treated with 60 Co-γ ray irradiation were used as the materials, and the changes of physiological indexes such as relative water content, relative electrical conductivity, proline content, chlorophyll content and root activity were measured under NaCl stress to evaluate and select varieties with strong salt tolerance. The sensitivity of PL-40-8 to salt stress was lower than that of the parental control Platinum(PL-CK). The relative water content, chlorophyll content and root activity of the mutants were significantly higher than PL-CK(P<0.05). The degree of cell membrane damage was less than PL-CK, and the increase in proline produced by salt damage was significantly lower than PL-CK(P<0.05). Therefore, the mutant material could be further selected and promoted as a salt-resistance grass species.
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