重金属镉胁迫对黑杨派无性系光合生理及生长的影响  

Effects of Cadmium Stress on the Growth and Photosynthesis of Aigeiros Clones

作　　者：姚俊修[1] 乔艳辉[1] 杨庆山[1] 仲伟国[1] 李庆华[1] 董玉峰[1] 李善文[1] 吴德军[1] YAO Jun-xiu;QIAO Yan-hui;YANG Qing-shan;ZHONG Wei-guo;LI Qing-hua;DONG Yu-feng;LI Shan-wen;WU De-jun(Key Laboratory for Genetics and Breeding in Forest Trees of Shandong Province,Shandong Academy of Forestry,Jinan 250014,Shandong,China)

机构地区：[1]山东省林业科学研究院山东省林木遗传改良重点实验室,山东济南250014

出　　处：《西北林学院学报》2020年第2期40-46,107,共8页Journal of Northwest Forestry University

基　　金：山东省重点研发计划项目(2017GNC11115);山东省林业科技创新项目(LYCX02-2018-08)。

摘　　要：为研究重金属Cd 2+胁迫对黑杨派无性系光合作用的影响,为日渐严重的土壤重金属污染治理提供依据。通过不同浓度Cd 2+(5、10、15、20、25 mg/L)对黑杨派5个无性系进行胁迫试验,测定其光合作用指标及生长量。研究发现,不同无性系因对Cd 2+抗性不一致导致其在不同浓度出现最高光合指标值。在不同浓度Cd 2+(0~25 mg/L)胁迫下,不同无性系P n随浓度升高均呈现先升高后降低的趋势。当Cd 2+达到最高浓度25 mg/L时,无性系L35的P n值较对照有上升趋势,其余无性系P n值均有下降趋势。各无性系G s随镉胁迫加强呈先降低后升高再降低趋势。在Cd 2+浓度达到25 mg/L时,各无性系G s值均有下降趋势,其中无性系中菏1号较对照下降最明显,其次为无性系鲁林16号、鲁林9号、L35、I-107。较P n、G s 2个指标无性系间C i变化不大,与CK相比,在Cd 2+浓度达到25 mg/L时,无性系中菏1号较对照下降最明显,其次为无性系L35、鲁林9号;而无性系I-107与鲁林16号较对照有上升趋势。T r在Cd 2+浓度为5 mg/L时出现降低,随着浓度增加,蒸腾作用出现先升高后降低的趋势。在Cd 2+浓度达到25 mg/L时,各无性系T r值均有下降趋势,其中无性系中菏1号较对照下降最明显;其次为无性系鲁林9号、鲁林16号、L35、I-107。无性系间的地径和苗高在Cd 2+胁迫下存在差异,苗高达到显著水平,而地径未达到显著水平,表明参试无性系苗高性状较地径对Cd 2+的胁迫更为敏感。相关分析表明,除了胞间CO 2浓度与净光合速率、苗高存在微弱的负相关外,其余性状间均存在不同程度的正相关。主成分分析发现,L35、鲁林9号和I-107的抗重金属Cd 2+能力较强,可为重金属Cd污染土壤的治理与修复提供试验材料。In order to study the effects of heavy metal stress on the photosynthesis of Aigeiros clones,and to provide a theoretical basis for solving the increasingly serious soil heavy metal pollution problem,seedlings of 5 Aigeiros clones were stressed under different concentrations of Cd 2+(namely,5,10,15,20,and 25 mg/L)in this test,and the photosynthesis indicators and growth were measured.It was found that different clones showed the highest photosynthetic index at different Cd 2+concentrations because their resistance to Cd 2+was different.With the increase of Cd 2+concentration in the range of 0 to 25 mg/L,the values of P n of different clones increased first and then decreased.When Cd 2+reached the highest concentration,compared with the control,the P n values of‘L35’showed an upward trend,while other clones showed a downward trend.The values of G s of different clones a trend of decrease-increase-decrease with the increase of cadmium stress.When the concentration of Cd 2+was 25 mg/L,the G s values of all clones showed a downward trend.Compared with the control,‘Zhonghe 1’decreased most significantly,followed by‘Lulin 16’,‘Lulin 9’,‘L35’and‘I-107’.Compared with P n and G s,there was little change in C i among clones.Compared with the control,when the concentration of Cd 2+was 25 mg/L,‘Zhonghe 1’decreased most obviously,followed by‘L35’and‘Lulin 9’,while‘I-107’and‘Lulin 16’showed an upward trend.When the concentration of Cd 2+was 5 mg/L,T r values decreased.With the increase of Cd 2+concentration,transpiration increased first and then decreased.When the concentration of Cd 2+reached 25 mg/L,the Tr values of all clones showed a downward trend,and‘Zhonghe 1’showed the most obvious decline compared with the control,followed by‘Lulin 9’,‘Lulin 16’,‘L35’and‘I-107’.There were differences in ground diameter and height among clones under the Cd 2+stress,the height reached a significant level,however,the ground diameter did not reach a significant level,which in

关 键 词：重金属 镉 黑杨派无性系 光合作用 

分 类 号：S718.43[农业科学—林学]